FAIMS-Selected Reaction Monitoring to Quantitative Protein Networks in Disease

描述（由申请人提供）：强大的研究技术（如质谱法）的进步使研究人员不仅能够对生物体或细胞器系统的蛋白质补体的许多变体进行编目，而且最近，使用定量蛋白质组学工具，在空间和时间上对细胞内的每种蛋白质组分进行特异性定量。FAIMS增强型TSQ-Vantage三重四极杆的收购对于ISB的研究基础设施至关重要，有助于开发和广泛传播下一代技术和仪器，以供从博士到博士的研究人员使用。学生和博士后在ISB和其他合作机构经验丰富的PI的。由实验学家和计算生物学家使用，与拟议的仪器收集的数据将通过识别和定量肽，其翻译后修饰，并最终通知研究人员的蛋白质组动态，负责生物体的形式和功能，提高蛋白质功能和行动的理解。FAIMS增强型TSQ-Vantage三重四极杆质谱仪的收购将大大提高我们在许多系统中特异性定量蛋白质的能力，这将使ISB研究人员能够推动该技术在识别对先天免疫反应，过氧化物酶体功能和DNA修复重要的蛋白质定量变化方面提供更深入的见解。这将通过筛选出化学背景噪音的能力来实现，特别是靶蛋白的靶蛋白质型特征，并以目前其他质谱仪无法实现的非常高的特异性和灵敏度对其进行定量。最后，该仪器将使研究人员能够组装给定系统的蛋白质互补的完整定量图谱，并从基因及其转录本的相互作用网络中进行推断。这项质谱技术的收购将有助于ISB及其合作伙伴目前正在进行的生物学研究的开发和完成。

DESCRIPTION (provided by applicant): Advances in powerful research technologies such as mass spectrometry have enabled researchers to not only catalogue the many variants of the protein complement of an organism or organelle system, but more recently, using quantitative proteomic tools, to specifically quantitate each protein component both spatially and temporally within the cell. The acquisition of the FAIMS-enhanced TSQ-Vantage triple-quadrupole is crucial to the research infrastructure at ISB for development and broad dissemination of next-generation technology and instrumentation to researchers ranging from Ph.D. students and post-docs to experienced PI's at ISB and other collaborating institutes. Used by experimentalists and computational biologists, the data collected with the proposed instrumentation will enhance the understanding of protein function and action through identification and quantitation of peptides, their posttranslational modifications and will ultimately inform researchers of proteome dynamics that are responsible for an organism's form and functions. The acquisition of a FAIMS-enhanced TSQ-Vantage triple-quadrupole mass spectrometer will substantially improve our ability to specifically quantitate proteins across many systems, which will enable ISB researchers to drive the technology to provide greater insights in identifying protein quantitative changes important to innate immune response, peroxisome function and DNA repair. This will be achieved through the ability to screen out chemical background noise, specifically target proteotypic signatures of target proteins and quantitate them with very high specificity and sensitivity not currently achievable with other mass spectrometers. Finally, this instrument will enable researchers to assemble a complete quantitative map of the protein complement of a given system and make inferences from the interacting network of genes and their transcripts. The acquisition of this mass spectrometry technology will aid in the development and completion of biological studies currently underway at ISB as well as with its collaborating partners.