ImageSteamX

描述（由申请人提供）：本NCRR共享仪器拨款申请的目的是为弗吉尼亚大学医学院的流式细胞术核心设施（FCCF）购买ImageStreamX成像流式细胞仪获得资金。FCCF是弗吉尼亚大学一个使用非常频繁的核心设施，它以服务收费的方式向大学社区提供与流式细胞术相关的服务，特别是向NIH资助的医学院研究人员提供服务。FCCF目前为来自27个不同部门95个pi实验室的200多名研究人员提供服务。FCCF于2005年购买了第一代ImageStream100，其资金来自医学院和SOM内的个人研究人员、部门和中心。这项技术很快被许多研究人员采用，并在早期发表了几篇重要的论文。弗吉尼亚大学已经成为使用这项技术的领导者，有19种出版物采用了这项技术，其中三种已经成为主要期刊的封面。利用这项技术的研究人员数量持续增长，目前有18个pi在他们的研究中使用这项技术。该仪器的一些常用应用包括细胞凋亡、酵母细胞周期、蛋白质易位、蛋白质共定位、形状变化分析、T细胞- apc偶联分析、细胞吞噬、罕见细胞鉴定、病毒载量/复制、细胞周期/有丝分裂研究以及功能性免疫细胞的形态和表型鉴定。在许多这些应用中，由于当前仪器的局限性，包括可以同时评估的颜色（标记）数量有限，采集速率缓慢，缺乏样品自动化，图像放大范围有限以及缺乏适当的激光激发线，研究受到了损害。在某些情况下，由于没有必要的激光激发或通道分离，研究人员无法将这种技术用于某些应用，例如FRET。随着ImagestreamX的引入，这些限制已经得到了解决，并将使我们的研究人员能够以更高效、更经济的方式推进项目。此外，一些研究人员将能够启动以前使用Imagestream 100无法实现的研究。

DESCRIPTION (provided by applicant): The purpose of this NCRR Shared Instrument Grant application is to secure funding to purchase an ImageStreamX imaging flow cytometer for the Flow Cytometry Core Facility (FCCF) at the University of Virginia School of Medicine. The FCCF is a very heavily used core facility at the University of Virginia and provides flow cytometry related services on a fee for service basis to the University community in general and particularly to NIH funded investigators in the School of Medicine. The FCCF currently serves over 200 investigators from the laboratories of 95 PIs in 27 different departments. The FCCF purchased the first generation ImageStream100 in 2005 with funds from the School of Medicine and contributions from individual investigators, departments and centers within the SOM. This technology was quickly adopted by a number of investigators that led to several key publications early on. The University of Virginia has become well known as a leader in the use of this technology and has had 19 publications employing the technology, three of which have been featured on the cover of major Journals. The number of investigators utilizing this technology has continued to grow with currently 18 PIs using this technology in their research. Some of the common applications for which the instrument has been used have been studies of apoptosis, yeast cell cycle, protein translocation, protein colocalization, shape change assays, T cell-APC conjugate analysis, trogocytosis, phagocytosis, rare cell identification, viral load/replication, studies of cell cycle/mitosis, and morphological and phenotypical identification of functional immune cells. In many of these applications, studies have been compromised by the limitations of the current instrumentation, including the limited number of colors (markers) that can be simultaneously evaluated, slow acquisition rates, lack of sample automation, limited range of image magnification and the lack of appropriate laser excitation lines. In some cases, investigators have been unable to take advantage of this technology for certain applications such as FRET because the necessary laser excitation or channel separation is not available. With the introduction of the ImagestreamX, these limitations have been addressed and will allow our investigators to move projects forward more efficiently and in a cost effective manner. In addition, several investigators will be able to initiate studies that were not previously feasible with the Imagestream 100.