CHARACTERIZATION OF THE TRANSCRIPTOME IN AN EMERGING MODEL FOR SOCIAL BEHAVIOR

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在本报告所述期间，从8种组织类型中产生了540万个平均阅读长度为250 bp的EST。作为上述测序方法的补充，使用与用于“长”EST测序的模板制备方案不同的模板制备方案，从4种组织类型产生了1亿~28-76 bp的EST。 通过将现有工具与定制计算机程序相结合，已经开发了序列分析管道。这个管道使我们能够快速地用定量指标来控制新数据，并构建累积的基因组装。序列数据现在定期加载到NCBI的Sequence Read Archive中，并免费向公众提供。 草原田鼠转录组数据的初步组装和分析表明，我们已经组装了来自15，000个基因的29 Mb序列。因此，我们正在对18，000个基因和50%的草原田鼠转录组进行部分测序。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During the reporting period, 5.4 million ESTs with an average read-length of 250 bp have been generated from 8 tissue types. As a complement to the above sequencing method, 100 million ~28-76 bp ESTs have been generated from 4 tissue types using a template preparation protocol different from that used for the 'long' EST sequencing. A sequence analysis pipeline has been developed by integrating existing tools with custom computer programs. This pipeline gives us the ability to rapidly QC new data with quantitative metrics and build cumulative gene assemblies. The sequence data is now being regularly loaded into the Sequence Read Archive at the NCBI and made freely available to the public. Preliminary assemblies and analysis of the prairie vole transcriptome data indicate we have assembled 29 Mb of sequence from 15,000 genes. We are thus well on our way to partially sequencing 18,000 genes and 50% of the prairie vole transcriptome.