DEVELOPMENT OF GENOMIC RESOURCES FOR THE PRAIRIE VOLE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在本报告所述期间，我们完成了草原田鼠BAC图书馆的特征描述，并发表了一篇介绍该图书馆的论文。利用随机BAC的末端测序，我们已经在300多个基因座中识别了2000多个SNPs。此外，我们还分离了包含20个行为相关基因(如催产素、血管紧张素、CRF及其受体、类固醇受体等)基因的BAC克隆，并正在准备一份描述这些基因座的手稿。利用SNPs和300多只草原田鼠的家系，我们创建了草原田鼠基因组的第一代连锁图谱。这一图谱将有助于组装草原田鼠的基因组。布罗德研究所目前正在对草原田鼠的基因组进行测序。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During the reporting period, we have completed the characterization of the prairie vole BAC library and published a paper describing the library. Using end sequencing of random BAC's we have identified more than 2000 SNPs in more than 300 loci. In addition, we have isolated BAC clones containing the genes for 20 behaviorally relevant genes (e.g. oxytocin, vasopress, CRF and their receptors, steroid receptor, etc), and a manuscript is in preparation describing these loci. Using the SNPs and a pedigree with more than 300 individual prairie voles we have created a first generation linkage map of the prairie vole genome. This map will be useful in assembling the prairie vole genome. The Broad Institute is currently sequencing the prairie vole genome.