DEVELOPMENT OF GENOMIC RESOURCES FOR THE PRAIRIE VOLE

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 在报告所述期间，我们完成了草原田鼠BAC文库的表征，并发表了一篇描述该文库的论文。 使用随机BAC的末端测序，我们已经在超过300个基因座中鉴定了超过2000个SNP。 此外，我们已经分离出含有20个行为相关基因（如催产素、血管加压素、CRF及其受体、类固醇受体等）的BAC克隆，并且正在准备描述这些基因座的手稿。使用SNPs和一个超过300个个体的草原田鼠谱系，我们已经创建了草原田鼠基因组的第一代连锁图谱。 这张图将有助于组装草原田鼠的基因组。 布罗德研究所目前正在对草原田鼠的基因组进行测序。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During the reporting period, we have completed the characterization of the prairie vole BAC library and published a paper describing the library. Using end sequencing of random BAC's we have identified more than 2000 SNPs in more than 300 loci. In addition, we have isolated BAC clones containing the genes for 20 behaviorally relevant genes (e.g. oxytocin, vasopress, CRF and their receptors, steroid receptor, etc), and a manuscript is in preparation describing these loci. Using the SNPs and a pedigree with more than 300 individual prairie voles we have created a first generation linkage map of the prairie vole genome. This map will be useful in assembling the prairie vole genome. The Broad Institute is currently sequencing the prairie vole genome.