NEUROGENETICS OF SOCIAL BEHAVIOR

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这个项目的目标是使用两种不同的方法来产生草原田鼠与干扰加压素受体信号。 第一种方法涉及创建表达靶向加压素受体基因的shRNA的转基因草原田鼠。在本报告期内，我们在体外筛选了几种潜在的shRNA，并确定了最佳序列。 我们还将这些shRNA序列放入慢病毒载体中，并表明它们减少了脑中加压素受体的结合。 我们现在准备将这些病毒注射到胚胎中。 第二种方法是创建锌指核酸酶蛋白，其靶向加压素受体序列以引起缺失。 与生产这些核酸酶的公司合作，我们已经确定了几种候选物，并将在注射到田鼠胚胎中之前开始在体外系统中筛选这些核酸酶。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to use two different approaches to generate prairie voles with disrupted vasopressin receptor signaling. The first approach involves creating transgenic prairie vole expressing shRNA's targeting the vasopressin receptor gene. During this reporting period, we have screened several potential shRNA's in vitro and have identified the optimal sequences. We have also put these shRNA sequences into a lentiviral vector and shown that they decrease vasopressin receptor binding in the brain. We are now ready to inject these viruses into embryos. The second approach is to create Zinc Finger Nuclease proteins that target the vasopressin receptor sequence to cause deletions. Working with the company that produces these nucleases, we have identified several candidates and will begin screening these in an invitro system before we inject into vole embryos.