BL3 AND FLOW CYTOMETRY CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 具体目标： 1.巩固和提高两个核心研究支助设施--BSL-3和FACS核心设施的运作效率。 2.促进和支持该机构招聘的新教员研究妇女健康方面的关切领域。 3.通过支持梅哈里医学院校园内的BSL-3设施，继续为艾滋病毒等感染性病原体的调查提供安全和适当的环境。 4.继续为利用流式细胞术方法研究卵巢癌和乳腺癌的分子生物学以及艾滋病毒感染和妇女健康研究的其他领域的研究提供技术基础设施支持。 刘彬东博士将担任核心PI，他将投入10%的精力进行这项活动。刘博士是BSL-3/FACS核心设施的现任主任，熟悉该设施的操作和维护。他在实验室经营着一家BSL-2+设施，并拥有艾滋病毒-1的研究经验。他将帮助教师、博士后和学生发展他们的研究方法，以实现他们的目标，并培训技术人员使用和应用新的FACS程序和BSL-3中的协议开发。 医学博士，高级研究助理(RA)。她接受过数据采集和分析方面的培训，用于FACSCalibur的日常操作。她协助调查人员进行涉及流式细胞术的项目。她能够帮助用户进行任何细胞分选，作为艾滋病毒和艾滋病相关传染病研究或其他需要细胞分选技术的项目的一部分。作为一名高技能的个人，她还接受了BSL-3程序的培训，并能够帮助和培训使用该设施的个人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SPECIFIC AIMS: 1. To consolidate and improve the efficiency of operation of two core research support facilities, the BSL-3 and the FACS Core Facility. 2. To facilitate and support the research of new faculty recruited to the institution who study areas of concern in women's health. 3. To continue to provide a safe and appropriate environment for the investigation of infectious agents such as HIV by supporting the BSL-3 facility on the Meharry Medical College campus. 4. To continue to provide technological infrastructure support to research utilizing methods in flow cytometry in the investigation of molecular biology of ovarian and breast cancer, as well as HIV infection and other areas of women's health research. Bindong Liu, Ph.D., will be the Core PI and he will commit 10% effort to the conduct of this activity. Dr. Liu is the current Director of the BSL-3/FACS Core Facility and is familiar with the operation and maintenance of it. He operates a BSL-2+ facility in his laboratory and has research experience with HIV-1. He will assist faculty, post docs and students develop their research methodology to achieve their objectives as well as training the technician on the use and application of new FACS procedures and protocol development in the BSL-3. Julia Shao, MD., Senior Research Assistant (RA). She is trained in data acquisition and analysis for day-to-day operation of the FACSCalibur. She assists investigators with projects involving flow cytometry. She is able to assist users in any cell sorting required as part of the HIV and AIDS-related infectious disease research or other projects requiring the cell sorting technology. As a highly skilled individual, she is also trained in BSL-3 procedures and is able to assist and train individuals who use that facility.