LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY & BIOIMAGING CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞生物学和生物成像（CBB）核心（http：//labs.pbrc.edu/cellbiology/index.htm）是根据COBRE项目PI和彭宁顿生物医学研究中心研究人员的具体需求组织的。 其目的是开发和提供最先进的生物成像方法，以研究活细胞和固定细胞和组织中生物过程的细胞和分子生物学，以及使用微型CT的体内生理过程。 CBB核心提供最先进的生物成像和分析仪器和支持，以促进COBRE项目PI和PBRC教师的数据收集和分析。 目前牛熊证的职员是大卫H.伯克谁担任主任，博士芭芭拉科扎克谁担任副主任和两个研究助理 女士考特尼·凯恩和德鲁里·英格拉姆先生 2006年9月，对彭宁顿COBRE的资助使细胞生物学和生物成像核心得以存在，它围绕着五个主要的分析平台组织起来：蔡司LSM-510 Meta光子共聚焦，直立式荧光显微镜和倒置式荧光显微镜，用于活细胞成像的扫描荧光计，以及FACS Caliber分析细胞分选仪。 最初两年的主要重点是加强我们现有的显微镜专业知识，并在流式细胞术，免疫组织化学，成像和图像分析领域开发新的专业知识。我们的总体目标是通过提供无缝访问这些技术来提高我们教师的竞争力。 牛熊证大致分为三部分  成像、组织学和标本制备以及流式细胞术。 在成像部分，核心有两个多光子共焦，一个全电动倒置系统的高速比率成像，一个直立电动系统的固定细胞或组织成像，多色激光TIRF装置，两个手动范围的常规检查载玻片或多孔板（明场和荧光），和一个数字病理切片扫描系统。 组织学和标本制备部分拥有四个低温恒温器，三个旋转切片机，一个自动组织处理器，组织包埋站，自动载玻片染色机/盖片机，自动IHC染色机和激光显微切割系统。 Core的流式细胞术部分包含用于四色分析的BD FACSCalibur和配备在BSL 2层流罩内的iCyt Reflection高速细胞分选仪。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Biology and Bioimaging (CBB) Core (http://labs.pbrc.edu/cellbiology/index.htm) was organized in response to the specific needs of COBRE Project PIs and researchers at the Pennington Biomedical Research Center. Its purpose is to develop and provide state of the art bioimaging approaches to study the cell and molecular biology of biological processes in live and fixed cells and tissues, as well as physiological processes in vivo using micro CT. The CBB Core provides state of the art bioimaging and analytical instrumentation and support to facilitate data collection and analysis by COBRE Project PIs and PBRC faculty. Currently the CBBC is staffed by Dr. David H. Burk who serves as director, Dr. Barbara Kozak who serves as associate director and two research associates Ms. Courtney Cain and Mr. Drury Ingram. Funding of the Pennington COBRE in September 2006 brought the Cell Biology and Bioimaging Core into existence and it was organized around five major analytical platforms: a Zeiss LSM-510 meta multiphoton confocal, an upright epifluorescence microscope and an inverted epifluorescence microscope, a scanning fluorometer for live cell imaging, and a FACS Caliber analytical cell sorter. A primary focus in the first two years was to enhance our existing microscopy expertise and development new expertise in the areas of flow cytometry, immunohistochemistry, imaging and image analysis. Our overall goal is to enhance the competitiveness of our faculty by providing seamless access to these technologies. The CBBC is loosely divided into three sections  imaging, histology & specimen preparation, and flow cytometry. Within the imaging section, the core has two multi-photon confocals, one fully motorized inverted system for high speed ratiometric imaging, an upright motorized system for fixed cell or tissue imaging, a multi-color laser TIRF rig, two manual scopes for routine examination of slides or multi-well plates (brightfield and fluorescence), and a digital pathology slide scanning system. The histology & specimen preparation section holds four cryostats, three rotary microtomes, an automated tissue processor, tissue embedding station, automated slide stainer/coverslipper, auto IHC stainer, and a laser microdissection system. The Core's flow cytometry section houses a BD FACSCalibur for 4 color analysis and an iCyt Reflection high speed cell sorter equipped within a BSL2 lamellar flow hood.