COBRE: U KS: CORE B: PROTEIN PURIFICATION GROUP

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 COBRE核心B蛋白质生产核心实验室位于堪萨斯大学Higuchi生物科学中心，并位于结构生物学中心。该实验室专注于原核和真核蛋白质的克隆，表达和纯化，为需要获得蛋白质用于研究的研究人员提供服务，为各个研究小组提供技术支持。该中心与生物核磁共振核心实验室、蛋白质结构核心实验室和高通量筛选实验室密切合作。核心B实验室为研究者提供一站式服务，包括方案开发;克隆;诱变;表达系统开发或改进;大规模或小规模蛋白质表达和/或纯化; Biacore结合研究;以及为这些领域的研究人员提供技术培训。该实验室还提供肽合成，分析和制备HPLC服务。工作人员可以在细菌或昆虫细胞系统中进行蛋白质表达。核心实验室拥有过量表达和纯化适当折叠和功能蛋白所需的设备和人员，其数量足以满足各种应用，包括功能和结合研究。设施中的主要设备包括：振荡培养箱;自动发酵（1-10 L规模）; AKTA纯化器系统和FPLC系统; Constant Systems液压细胞破碎机和Biacore 3000表面等离子体共振系统。此外，实验室还根据蛋白质的理化性质和研究者的需求，提供蛋白质生成和分析方面的建议。工作人员包括一名主任和两名全职技术员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE Core B Protein Production Core Laboratory is hosted within The University of Kansas Higuchi Bioscience Center and is housed in the Structural Biology Center. The lab focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for researchers who need to obtain proteins for their research, which adds technical support to individual research groups. The Core works closely with the Bio-NMR Core Laboratory, the Protein Structure Core Laboratory, and the High Throughput Screening Laboratory. The Core B laboratory provides a one-stop menu of services to investigators, including protocol development; cloning; mutagenesis; expression system development or improvement; large or small scale protein expression and/or purification; Biacore binding studies; and technical training for researchers in any of these areas. The laboratory also provides services in peptide synthesis, and analytical and preparative HPLC. The staff can perform protein expression in either bacterial or insect cell systems. The core laboratory possesses the equipment and personnel required for overexpression and purification of properly folded and functional proteins in quantities sufficient for a variety of applications, including functional and binding studies. The major equipment in the facility includes: Shaker-incubator; Automated fermentation (1-10 L scale); AKTA Purifier system and FPLC system; Constant Systems Hydraulic Cell Disruptor, and a Biacore 3000 Surface Plasma Resonance system. In addition, the laboratory provides advice on the generation and analysis of proteins depending on their physicochemical properties and the needs of the investigator. The staff consists of a Director and two full time technicians.