QUANTIFICATION OF METABOLITES WITH EXCHANGEABLE PROTONS USING SPIN-LOCK MRI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近在成像技术方面做了很多工作，这种技术利用质子的化学交换来 使磁共振成像对蛋白质及其周围环境的浓度敏感。 旋转标架中的自旋晶格弛豫是一种成像技术，它依赖于 质子交换，但尚未应用于基于其化学物质的特定代谢物成像 交换属性。到目前为止，还没有研究将T1用于化学物质的对比 质子交换。T_1？化学交换效应随静磁场的变化呈二次曲线变化。 因此，T1？在探测交换介导的高场中有可能提供更高的灵敏度 核自旋系统中的相互作用。T1？MRI的这种更高的灵敏度可以使检测到 低浓度(~1 mM)具有质子交换部位的内源代谢物。 我们的假设是，基于TIP的MRI将不太容易受到线条形状变化的影响，并提供 在检测具有可交换质子的代谢物时具有更高的灵敏度。这样做的目的是 子项目是开发一种灵敏而稳健的方法来量化和 表现出质子与水的化学交换的代谢物。 研究目标： 1.开发一种利用T1？MRI技术创建图像的方法 用可交换的质子对比和量化代谢物。 2.测定浓度、pH值、恒磁场、B1场强和 饱和时间对化学交换和随后的T1？对比 3.为了证明我们的模型可以可靠地用来量化空气中的 动物模型和人类中具有可交换质子的代谢物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much work has been done recently on imaging techniques that utilize chemical exchange of protons to make MR imaging sensitive to concentrations of proteins and their immediate environments. Spin-lattice relaxation in the rotating frame (T1¿) is an imaging technique which is dependent on the exchange of protons but has yet to be applied to imaging specific metabolites based on their chemical exchange properties. There have been no studies to date using T1¿ for contrast in the chemical exchange of protons. T1¿ Chemical exchange effects vary quadratically with the static magnetic field. Therefore, T1¿ potentially offer higher sensitivity at higher fields in probing exchange mediated interactions in nuclear spin systems. This higher sensitivity of T1¿ MRI may enable detection of endogenous metabolites that possess a proton exchange site at low concentrations (~1 mM). Our hypothesis is that Tip based MRI will be less susceptible to line shape changes and provide higher sensitivity in detecting metabolites with exchangeable protons. The purpose of this subproject is to develop a sensitive and robust method to quantify the concentration of and metabolites exhibiting a chemical exchange of protons with water using T1¿. Study Aims: 1. To develop a method to that utilizes T1¿ MRI techniques that can be used to create image contrast and quantify metabolites with exchangeable protons. 2. To determine the effect that concentration, pH, static magnetic field, B1 field strength and saturation time have on the chemical exchange and subsequent T1¿ contrast 3. To demonstrate that our model can be reliably used to quantify the concentration of metabolites with exchangeable protons in animal models and humans.