STRUCTURAL INVESTIGATION OF PROTEINS IN THE ENDOCYTIC PATHWAY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们对神经元突触的细胞生物学感兴趣，重点是突触小泡，这是一种储存和释放神经递质的特殊分泌细胞器。我们的目标是学习有关突触传递的知识，但也要增进外吞和内吞领域的一般知识。在这些过程中，蛋白质机器被用来通过膜重塑，通过改变膜的组成和变形来在细胞内运输特定的货物。为了研究这一过程，我们采取了结构方法，以了解这些蛋白质复合体是如何形成的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the cell biology of neuronal synapses, with emphasis on synaptic vesicles, the special secretory organelles that store and release neurotransmitters. Our goal is to learn about synaptic transmission but also to advance general knowledge in the field of exo- and endocytosis. In these processes, protein machineries are used to traffic specific cargos within the cell through membrane remodeling, by both changing the composition of the membrane and by deforming the membrane. In order to study this process, we are taking a structural approach in order to understand how these protein complexes are formed.