CRYSTALLOGRAPHIC STUDIES OF LARGE MACROMOLECULAR ASSEMBLIES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 蛋白质和核酸的结构研究以及它们所形成的复合物是我们正在进行的研究的主题，这些复合物参与了克里克分子生物学中心法则的执行。 所追求的结构包括细菌复制体组分之间的复合物-与引发酶和DNA复合的DnaB解旋酶以及与Tau片段的复合物;大肠杆菌RNA聚合酶与DNA复合; CAP无配体和CAP与cAMP结合DNA与RNA聚合酶;新型氨酰-tRNA合成酶与底物复合; 70 S核糖体与底物、因子或抗生素复合的结构; 50 S核糖体亚基与抗生素之间的复合物;真核核糖体的结构研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural studies of proteins and nucleic acids and the complexes they make that are involved in executing Crick's Central Dogma of Molecular Biology are the subjects of our ongoing studies. The structures being pursued include complexes between the components of the bacterial replisome - DnaB helicase complexed with primase and DNA as well as complexes with a fragment of Tau; E. coli RNA polymerase complexed with DNA; CAP without ligands and CAP with cAMP bound to DNA with RNA polymerase; novel aminacyl-tRNA synthetases complexed with substrates; structures of the 70S ribosome complexed with substrates, factors, or antibiotics; complexes between the 50S ribosomal subunit and antibiotics; structural studies of the eukaryotic ribosomes.