REGULATION OF THE SIGNALING PHOSPHOLIPID, PHOSPHATIDYLINOSITOL 3,5 BIS PHOSPHATE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 磷脂酰肌醇多磷酸（PPIs）最近成为膜运输途径的关键调节剂。我们最近发现，PPI，磷脂酰肌醇3，5二磷酸（PI 3，5）P2，对神经系统的正常功能特别重要。 我们发现，（PI 3，5）P2生成过程中非常微小的缺陷是ALS的一个重要风险因素。 此外，我们最近发现已知调节PI 3、5 P2的五种蛋白质被磷酸化。 本项目的总体目标是确定磷酸化位点，然后使用遗传方法确定磷酸化如何调节PI 3，5 P2水平

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phosphatidylinositol polyphosphates (PPIs) have recently emerged as key regulators of membrane trafficking pathways. We recently discovered that the PPI, phosphatidylinositol 3,5 bis phosphate (PI3,5)P2, is particularly critical for normal function of the nervous system. We found that very minor defects in the generation of (PI3,5)P2 are a significant risk factor in ALS. Moreover we recently found that the five proteins known to regulate PI3,5P2 are phosphorylated. The overall goal of this project is to determine the phosphorylation sites, and then to use a genetic approach to determine how phosphorylation regulates PI3,5P2 levels