MEASUREMENT OF ANALYTES IN WHOLE BLOOD AND SERUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 临床相关血液分析物水平的快速和可靠测量对于医生在评估患者的治疗选择时是非常宝贵的。 目前的临床实验室测试涉及样品制备、使用昂贵的试剂，并且是时间密集型的。 拉曼光谱是有吸引力的，因为它提供了特异性，并可以同时测量多个分析物。 在过去的几年中，近红外拉曼已被证明能够检测在毫摩尔范围内的多种血液分析物。 该可行性研究旨在重新评估微摩尔范围内的NIR拉曼光谱检测限，该范围涵盖各种临床重要的血液分析物。 为了提高检测限，在解释拉曼光谱时遇到的技术挑战-例如样品之间的浊度变化和拉曼光谱中的发光背景-将被处理，化学计量学算法的新应用也将被探索。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rapid and reliable measurements of the levels of clinically-relevant blood analytes are invaluable to physicians when making assessments about a patient's treatment options. Current clinical laboratory tests involve sample preparation, the use of expensive reagents, and are time-intensive. Raman spectroscopy is attractive in that it offers both specificity and can measure multiple analytes at once. In the past years, NIR Raman has been proven to be able to detect multiple blood analytes in the millimolar range. This feasibility study seeks to re-evaluate NIR Raman spectroscopy detection limits in the micromolar range which covers a variety of clinically-important blood analytes. To improve the detection limits, technical challenges encountered in the interpretation of Raman spectra- such as turbidity variation across samples and luminescence background in Raman spectra- will be dealt with and novel applications of Chemometrics algorithms will also be explored.