MASS SPECTROMETRY OF SIGNALLING LIPIDS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 合作者希望对细胞信号脂质进行质谱分析。他们正在评估磷脂酰肌醇二磷酸(PIP2)在质膜上的分布，这与IgE受体介导的信号转导有关。他们已经获得了一些很好的初步数据，并与麦克拉弗蒂教授发表了一篇论文，但这是他通常研究之外的研究，所以他让调查人员参考了BUSM资源。他们想要使用的方法与一些已发表的作品相似，但这里提出的工作的难度更大。他们寻求的主要结果是将他们目前的生化数据与质谱图中观察到的某些峰相关联。量化不同的PIP2物种也应该是非常有趣的。BUSM已经获得了一些初步数据，该学生计划访问以参与进一步的分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The collaborators would like to carry out mass spectrometric of cell signaling lipids. They are evaluating phosphatidyl inositol bis phosphate (PIP2) distributions in plasma membranes related to IgE receptor mediated signaling. They have already obtained some nice preliminary data and have published a paper with Prof. McLafferty, but this are of research is outside of his usual research, so he has referred the investigators to the BUSM Resource. The approach theywould like to use paralles that of some published work, but the difficulty of the work proposed here is greater. The primary result they seek is correlating their present biochemical data to certain peaks observed in the mass spectra. It also should be very interesting to quantify different PIP2 species. Some initial data has been obtained at BUSM and the student plans to visit to participate in further analyses.