IMMUNOHISTOCHEMICAL IDENTIFICATION OF NONHUMAN PRIMATE ANTIGENS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了提高我们的诊断病理学能力，我们系统地评估了针对人类细胞/组织和感染剂的商业可用抗体，以应用于NEPRC中的非人类灵长类物种。抗体被选择用于诊断和/或实验病理学的潜在应用，以及在速冻或福尔马林固定的石蜡包埋组织中的反应性。此外，比较病理学分部每年都会收到多项请求，要求检测抗体并为我们分部的调查人员、其他非传染性疾病控制中心分部和合作的调查人员制定IHC方案。因此，研究和开发识别非人灵长类动物免疫组织化学抗体是分子病理学核心的一项重要活动，并有助于开发出在猕猴组织中持续发挥作用的各种IHC抗体库。在一项研究中，我们能够识别出4种猕猴的21种抗原中的21种，以及新世界猕猴的21种抗原中的17种。在2005年，我们开发了21种新抗体的免疫组织化学方案，这些抗体在猴子组织中具有交叉反应。比较病理学分部还对研究人员、技术人员、博士后研究员和研究生进行免疫组织化学方法培训。与艾滋病有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to improve our diagnostic pathology capabilities, we systematically evaluate commercially available antibodies directed against human cells/tissues and infectious agents for application in the nonhuman primate species housed at the NEPRC. Antibodies are selected for potential applications in diagnostic and/or experimental pathology, and for reactivity in either snap-frozen or formalin-fixed, paraffin-embedded tissues. In addition, the Division of Comparative Pathology receives multiple requests each year to test antibodies and develop IHC protocols for investigators in our Division, other NEPRC Divisions, and collaborating investigators. Thus, research and development to identify antibodies for immunohistochemistry in nonhuman primates is an important activity of the Molecular Pathology Core, and has contributed to the development of a diverse repertoire of IHC antibodies that work consistently in macaque tissue. In one study we were able to identify 21 of 21 antigens in 4 macaque species and 17 of 21 in New World monkeys, and during 2005, we developed immunohistochemistry protocols for 21 new antibodies that were cross-reactive in monkey tissues. The Division of Comparative Pathology also trains investigators, technicians, postdoctoral fellows and graduate students in immunohistochemistry methods. AIDS related.