ACCOMMODATING INTRAOCULAR LENSES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：研究中枢刺激对恒河猴人工晶状体调节能力的影响。 将电极植入6只恒河猴的Edinger Westphal核内，以实现中枢调节。镜片物质被NuLens设计的人造镜片取代。用折射法和超声生物显微镜测定这些晶状体在中枢刺激后适应、改变形状和位置的能力。刺激诱导调节度数为8.0度。因此，这些镜片有可能逆转随着年龄增长的老花眼的发病。已确定四只因人工晶状体手术和严重的角膜混浊而发炎的动物需要在一只眼或双眼接受角膜移植。对供体猴进行定位，并进行角膜移植。移植4个月后，裂隙灯检查(SLE)显示两只动物没有排斥迹象，但所有角膜似乎都略显模糊。这种模糊在双侧治疗的猴子中更加明显。尼尔·巴尼博士是我们的角膜专家之一，自这些动物进行移植手术以来，他对它们进行了多次检查。他认为，这些动物的角膜会保持轻微的模糊，但已经成功地耐受了移植过程，外观和行为与改善的视力一致。 由于没有收集到两只角膜模糊程度更明显的动物的可测量住宿数据，这些动物被实施了安乐死。 三只恒河猴在人工晶状体植入后至少8个月内保持3到8个屈光度的调节。每季度进行一次实验，以记录NuLens IOL的功能。可能会有另外两只恒河猴接受电极植入，随后是下一代NuLens IOL。 这项研究使用了WNPRC Research Services。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To determine the accommodative ability of intraocular lenses in rhesus monkeys following central stimulation. Electrodes were implanted into the Edinger Westphal nucleus of 6 rhesus monkeys so that accommodation could be stimulated centrally. The lens substance was replaced with artificial lenses designed by NuLens. The ability of these lenses to accommodate, change shape and position after central stimulation was determined using refractometry and ultrasound biomicroscopy. Accommodation of 8.0 diopters was induced during stimulation. Thus, these lenses have the potential to reverse the onset of presbyopia with age. It was determined that four animals with inflammation due to the intraocular lens surgery and severe corneal opacification required a corneal transplant in one or both eyes. Donor monkeys were located and corneal transplants were performed. Four months post transplant, slit lamp examination (SLE) revealed no rejection signs in two animals, but all corneas appeared slightly hazy. The haziness was more pronounced in the bilaterally treated monkeys. Dr. Neal Barney, one of our corneal specialists, examined these animals numerous times since their transplant procedure. He felt that these animals would maintain slightly hazy corneas, but had successfully tolerated the transplant procedure with an appearance and behavior consistent with improved vision. Since no measurable accommodation data was gathered in the two animals with more pronounced cornea haziness, those animals were euthanized. Three rhesus monkeys maintain accommodation from 3 to 8 diopters for at least eight months post IOL insertion. Experiments are conducted quarterly to document the NuLens IOL functionality. Potentially two additional rhesus monkeys will receive an electrode implant followed by the next generation NuLens IOL. This research used WNPRC Research Services.