Importance of pulmonary venous electrophysiology in the development of atrial fibrillation

（ii）移植排斥反应可以通过CTLA4-Fc的表达来抑制，CTLA4-Fc是一种阻断T细胞共刺激增强同种异体移植接受的试剂（Transplantation 2000 69:1806）。高水平表达超过100天预计与最佳移植物接受相关。我们使用Kunjin在体内数月无炎症地表达β -半乳糖苷酶的能力说明了这种方法的潜力（CIB ref 15）。最初，我们打算将P815细胞注射到C57BL-6中，这些细胞通常在几天内被排斥。相比之下，具有昆津复制子介导的CTLA4-Fc表达的P815细胞应该存活较长时间。使用FACS和抗h -2d抗体很容易监测移植物存活。

(ii) Transplant rejection can be inhibited by expression in the graft of CTLA4-Fc a reagent that blocks T cell co-stimulation enhancing allo-graft acceptance (Transplantation 2000 69:1806). High-level expression for over 100 days is expected to correlate with optimal graft acceptance. Our ability to use Kunjin to express beta galactosidase for several months in vivo without inflammation illustrates the potential for this approach (CIB ref 15). Initially we intend to use P815 cells injected i.p. into C57BL-6, where they are usually rejected within a few days. In contrast, P815 cells with Kunjin replicon-mediated CTLA4-Fc expression should survive for an extended period. Graft survival is easily monitored using FACS and anti-H-2d antibodies.