Tissue/Pathology Core

该组织/病理学核心为参与宫颈癌孢子和其他合作研究工作的研究人员提供了充分表征的人类生物标本。在第一个资助期内，该核心收集了超过78，000份来自超过3524名宫颈癌患者的个人等分试样，迄今已分发了6332份等分试样。这包括迄今为止来自JHU乳腺癌和头颈部孢子的临床试验和两项流行病学研究的838名患者的28，000多份等分试样，无需额外费用。除了对复杂样品采集的需求不断增长外，我们的 研究者依赖于专家病理学的支持，以确保适当的组织制备和表征所选择的研究。该核心提供了这样的专家病理学评价的标本和技术支持。具体而言，这一核心继续：1。在不损害患者护理或保密性的情况下，获得知情同意并从患者身上采集标本进行转化研究; 2.收集宫颈癌和癌前病变，以及患者的正常组织，包括那些参加SPORE项目临床试验的患者; 3.收集病人的血液、分泌物及脱落细胞（例如子宫颈刮屑），包括参加SPORE临床试验的病人; 4.按照SOP处理和储存临床标本，以满足所有SPORE研究者的要求; 5.将样本信息输入 中央数据库系统和跟踪样本分配和运输; 6.表征组织标本的起源部位、病理分级和分期以及肿瘤和间质组织的比例; 7.使用明确定义的机制来优先向研究者分发所需标本 约翰霍普金斯孢子内外; 8.尽可能以低成本和高效率的方式及时提供质量受控的样本; 9.在按服务收费的综合癌症中心核心区提供用于组织学和病毒学分析的标本，例如免疫组织化学染色、原位杂交、组织微阵列、HPV检测和分型、免疫表型或激光捕获显微切割 设施; 10.支持免疫分析的开发和实施。将使用CaTissue（一种CaBIG投诉数据库）跟踪样本，该数据库可供我们在UAB和UC的项目访问。该核心的活动将与行政/临床核心A、生物统计学和数据管理核心B以及免疫学核心D的活动整合，以确保标本和临床信息得到适当的编目和传播。

This Tissue/Pathology Core provides well-characterized human biological specimens to researchers participating in this Cervical Cancer SPORE and other collaborative research efforts. In the first funding period this core collected over 78,000 individual aliquots from over 3524 patients enrolled by the SPORE in Cervical Cancer and distributed 6332 aliquots to date. This includes over 28,000 aliquots from 838 patients to date from a clinical trial and two epidemiologic studies of the JHU Breast Cancer and Head and Neck SPOREs at no additional cost. In addition to the growing need for sophisticated sample acquisition, our investigators depend on expert pathology support to ensure proper tissue preparation and characterization for selected studies. This Core provides such expert pathologic evaluation of specimens and technical support. Specifically, this core continues to: 1. Obtain informed consent and collect specimens from patients for translational research without compromise of patient care or confidentiality; 2. Collect cervical carcinoma and pre-malignant lesions, as well as normal tissue from patients, including those enrolled in clinical trials for the SPORE projects; 3. Collect blood, secretions and exfoliated cells (e.g. cervical scrapes) from patients, including those enrolled in clinical trials for the SPORE projects; 4. Process and store clinical specimens following SOPs to address the requirements of all SPORE investigators; 5. Input specimen information into central database system and track specimen distribution and transport; 6. Characterize tissue specimens with respect to site of origin, pathologic grading and staging, and proportion of neoplastic and stromal tissue; 7. Use well-defined mechanisms for prioritization of the distribution of requested specimens to investigators within and external to the Johns Hopkins SPORE; 8. Provide quality-controlled specimens in a timely fashion as inexpensively and efficiently as possible; 9. Route specimens for histologic and virologic analyses e.g. immunohistochemical staining, in situ hybridization, tissue microarraying, and HPV testing and typing, immunophenotyping, or laser-capture microdissection in fee-per-service Comprehensive Cancer Center core facilities; 10. Support the development and implementation of immunologic assays. The samples will be tracked using CaTissue, a CaBIG complaint database, web-enabled for access by our projects at UAB and UC. The activities of this Core will be integrated with those of the Administrative/Clinical Core A, the Biostatistics and Data Management Core B and the Immunology Core D to ensure that specimens and clinical information are appropriately catalogued and disseminated.