Analytical, Administrative and Animal/Reagent Support

在协调计划项目内进行研究的优势是能够创建核心设施，以(I)高效和经济地共享资源和行政支持，(Ii)为计划的科学目标提供基础设施，(Iii)提供传播结果和促进小组内讨论的机制，以及(Iv)协调与外部调查人员的互动，以获得关键评估、技术建议和智力投入，以保持工作的正轨和进展 该领域的前沿技术。为此，将创建一个分析、管理和动物/试剂核心(核心A)，并由Christine Gall博士指导。核心A将解决4个具体目标。目标1将维持显微镜、电生理学、行为和分析功能的分析核心。项目1-4中的研究包括分析海马片的长时程增强(LTP)，定位肌动蛋白的信号，调节内源性BDNF蛋白含量，以及评估非监督学习的治疗效果。这将使用分析核心设施和人员进行显微图像分析、电生理学、行为分析(无监督学习)和蛋白质分析。目标2将是支持动物和试剂功能。核心人员将协调试剂和Ampakines的采购，支持用于UCL项目的小鼠群体，并执行基因分型。目标3将管理项目实验室之间的合作和研究整合，并提供来自内部和外部咨询委员会(行政)的投入。这包括协调两项研究活动 在获得关键分析设施(例如，显微镜、电生理和行为设施和工作人员)和计划项目协作会议的项目中。核心还将与内部和外部咨询委员会以及研讨会发言人举行会议。目标4是为所有方案调查员(行政)提供一般行政支助和计算机协助。这包括对赠款管理的一般行政支持，协调研讨会，监督核心A的动物使用，监督核心人员，以及维护计划活动的计算机服务器。总体而言，核心设施和职能将在项目内提供关键的研究整合，并将支持对实现项目目标至关重要的技术平台。

An advantage of conducting research within a coordinated Program Project is the ability to create core facilities to (i) efficiently and economically share resources and administration support, (ii) provide infrastructure for the scientific objectives of the program, (iii) provide mechanisms for disseminating results and facilitating discussions within the group, and (iv) coordinate interactions with outside investigators to obtain critical evaluation, technical advice and intellectual input to keep the work on track and at the cutting edge of technologies in the field. To this end an Analytical, Administrative and Animal/Reagent Core (Core A) will be created and directed by Dr. Christine Gall. Core A will address 4 specific aims. Aim 1 will be to maintain an Analytical Core for Microscopy, Electrophysiology, Behavior and Assay functions. Studies within Projects 1-4 entail analyses of long term potentiation (LTP) in hippocampal slices, localized signaling to actin, modulating endogenous BDNF protein content, and evaluation of treatment effects on unsupervised learning. This will be accomplished using Analytical Core facilities and personnel for microscopy-image analysis, electrophysiology, behavioral analysis (unsupervised learning) and protein assays. Aim 2 will be to support Animal and Reagent functions. Core personnel will coordinate purchases of reagents and ampakines, support mouse colonies employed for UCl Projects, and perform genotyping. Aim 3 will be to manage collaborations and integration of research among Project laboratories, and provide input from internal and external advisory boards (Administration). This includes coordinating both research activities among the projects for access to key analytical facilities (e.g., microscopic, electrophysiological and behavioral facilities and staff) and Program Project collaborative meetings. The Core will also convene meetings with Internal and External Advisory Boards, and seminar speakers. Aim 4 is to provide general administrative support and computer assistance for all program investigators (Administration). This includes general administrative support for grants management, coordinating seminars, oversight of animal use in Core A, supervision of Core personnel, and maintenance of computer servers for Program activities. Overall the Core facilities and functions will provide critical integration of research within the Projects and will support technical platforms that are critical for reaching project goals.