Mouse Core

Mouse Core的目的是提供一个集中的面向服务的设施， 每个参与项目的实验室生产和维护转基因和基因破坏小鼠。鼠标 核心还将通过支持两个肺损伤模型的基础设施来服务于该计划的项目 这些项目将广泛使用的方法，即接触香烟烟雾和仙台病毒 感染，监督每个模型的标准化协议的使用，并确保法规遵从性 通过一个集中的设施。Mouse Core对仙台感染模型的支持也将是 提供用于在形态学核心（气液界面）内生成的体外细胞培养模型 气管上皮细胞培养物和Clara细胞/肺泡上皮细胞培养物）。更以 与这些模型相关联，Mouse Core将通过测量肺功能来支持个人项目。 使用BUXCO系统测量生理参数，例如体内阻力和顺应性。因此 鼠标核心将是这个计划项目赠款的重要组成部分。鼠标的主要组成部分 核心，包括转基因/基因敲除生产单位，吸烟设施和仙台感染模型 每一个都有我们动物设施内的专用空间提供支持，并且设备齐全。当老鼠 在上一个资助周期，核心项目是该计划项目赠款的一部分，该核心项目最初是 1995年在我们的肺部部门开发，并被项目研究者广泛使用， 多年来，该应用程序的核心董事。它由具有集体延伸轨道的人员组成 在这方面的专业知识和生产力记录。鼠标核心负责生成超过 100个转基因和敲除品系，每个品系具有多个创始者/嵌合体。许多老鼠 将进一步促进项目之间的协作互动 Investigators.由于该计划将与其他两个项目共同支持转基因/基因敲除生产单位， 美国国立卫生研究院中心的赠款，这将使我们能够实现规模经济和降低生产成本，每只老鼠 生成的.

The purpose of the Mouse Core will be to provide a centralized service-oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The Mouse Core will also serve the projects of this Program by supporting the infrastructure of two lung injury models that will be extensively utilized by the projects, namely exposure to cigarette smoke and Sendai virus infection, overseeing the use of standardized protocols of each model and ensuring regulatory compliance through one centralized facility. Support of the Sendai infection model by the Mouse Core would also be provided for use in in vitro cell culture models generated within the morphology core (air liquid interface cultures of tracheal epithelial cells, and Clara cell/alveolar epithelial cell cultures). Furthermore, in association with these models, the Mouse Core will support individual projects by measuring pulmonary physiological parameters such as resistance and compliance in vivo using the BUXCO system. Thus, the Mouse Core will be an important part of this Program Project Grant. The major components of the Mouse Core, including the transgenic/knockout production unit, the smoking facility, and the Sendai infection model are each supported by dedicated space within our animal facility and are fully equipped. While the Mouse Core was part of this Program Project Grant during the previous funding cycle, this core was initially developed in our Pulmonary Division in 1995 and has been extensively utilized by Project Investigators and Core Directors on this application for many years. It is staffed by personnel with a collective extenisive track record of expertise and productivity in this area. The Mouse Core has been responsible for generating over 100 transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will further serve to facilitate collaborative interactions amongst Project Investigators. As this Program would jointly support the transgenic/knockout production unit with two other NIH center grants, this will allow us to realize an economy of scales and lower production costs per mouse generated.