Mouse Physiology

1.核心功能 小鼠生理学和表型： 小动物生理学核心将为PPG中的所有四个项目提供两项主要服务：1) 小鼠心肌肥厚和心力衰竭的病理生理模型，以及2)完整和 野生型和转基因小鼠的综合表型。 鼠标模型系统包括： A)机械引起的压力超负荷肥大和心力衰竭 主动脉缩窄(TAC)，包括慢性和间歇性。 B)心肌梗死 C)心力衰竭的遗传模型：肌肉LIM蛋白敲除(MLP[-/-])和钙调素(CSQ) 过度表达。 D)游泳和跑步运动。 小鼠的表型将包括： A)通过系列超声心动图对清醒小鼠体内心脏功能的深入分析。 B)通过压力-体积(PV)环分析活体测量固有的心肌收缩能力。 C)利用应力-应变关系计算心肌收缩末期应力。 D)导管衍生血流动力学在体内的PAR反应性。 E)有创和无创血压记录和通过编程跑步机进行的运动能力 测试。 F)动态心电遥测。 G)体外测量分离的心肌细胞的收缩能力。 这个核心的领导者已经参与了无数外科手术和遗传模型的开发。 心脏肥厚和心力衰竭。PI的实验室在广泛的领域拥有相当多的专业知识 一系列用于小鼠表型鉴定的方法。国际和平研究所还参与了一项 心力衰竭的遗传和外科模型的数量。例如，显微外科手术和 横动脉缩窄致压力超负荷性肥厚的血流动力学评价 由PI开发，现在是世界各地许多实验室使用的标准方法。

1. Core Functions Mouse Physiology and Phenotyping: The small animal physiology core will provide two major services to all four of the projects in this PPG: 1) pathological and physiological models of cardiac hypertrophy and heart failure in mice, and 2) complete and comprehensive phenotyping of wild type and genetically modified mice. Mouse Models systems include: A) Mechanically- induced pressure overload hypertrophy and heart failure using the technique of transverse aortic constriction (TAC), both chronic and intermittent. B) Myocardial Infarction C) Genetic models of heart failure: muscle LIM protein knock out (MLP[-/-]) and calsequestrin (CSQ) overexpression. D) Swimming and running exercise. Mouse Phenotyping will include: A) An in-depth analysis of in vivo cardiac function by serial echocardiography in conscious mice. B) In vivo measurements of intrinsic myocardial contractility by pressure-volume (PV) loop analysis. C) In vivo end-systolic myocardial stress using stress-strain relations. D) In vivo PAR responsiveness by catheter derived hemodynamics. E) Invasive and non-invasive blood pressure recordings and exercise capacity by programmed treadmill testing. F) Ambulatory electrocardiographic telemetry. G) Ex vivo measurements of isolated myocyte contractility. The leaders of this core have been involved in the development of numerous surgical and genetic models of cardiac hypertrophy and heart failure. The Pi's laboratory has considerable expertise in a wide-ranging array of methodologies for the phenotyping of mice. The PI has also been involved in the development of a number of genetic and surgical models of heart failure. For example, microsurgical procedures and hemodynamic evaluation of pressure overload hypertrophy induced through transverse aortic constriction developed by the PI, is now a standard method used by many laboratories around the world.