PARTNERSHIPS FOR BIOMEDICAL RESEARCH IN ARKANSAS: SCIENCE RESEARCH CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 科学研究核心 阿肯色州INBRE提议继续其科学研究核心，这将包括由两个领先机构的三个科学研究机构提供的服务。这些核心设施将为全州的调查人员提供现代生物技术的三个关键领域-蛋白质组学、数字显微镜以及DNA损伤和毒理学-所需的仪器和专业知识。之所以选择这三个领域，是因为每个设施的技术专长，特别是仪器设备并不常见，即使在普通的、资金充足的研究实验室，特别是在四年期的PUI。选择这三个设施是为了满足13位阿肯色州INBRE项目负责人的具体需求(见表11)。有了这些特定研究的技术，研究人员就可以在自己的实验室继续和扩展研究项目。这些设施的使用应该有助于研究人员获得必要的初步数据，以便申请外部研究资金。阿肯色州INBRE对科学研究核心及其三个设施的支持主要是为了保证PUI调查人员(包括教师和学生)访问这些设施。这种支持不足以完全维持这些设施，这些设施从其他赠款资金来源及其所在机构(UAMS和UAF)获得更多、更实质性的支持。 下文将详细介绍这三个设施，它们的目标是为下列目的提供专门知识和设备： +蛋白质组学：使研究人员能够使用最先进的仪器研究蛋白质结构和功能； +数码显微镜：使研究人员能够定位细胞和组织中的大分子，从而更好地评估它们在正常和疾病状态下的功能；以及 +DNA损伤和毒理学：使研究人员能够将DNA损伤和毒性分析(如细胞损伤和死亡)纳入他们对正常和患病细胞和组织的研究中。 每个科学研究核心设施还将致力于在合作伙伴和非合作伙伴PUI中教育和培训阿肯色州INBRE的教职员工和学生，掌握每个设施特有的尖端技术。因此，所有设施都将提供与现有仪器和专门知识相关的讲习班或演示会。这些活动将由所有科学研究核心设施的领导人组织，并由全州行政核心进行宣传。 科学研究核心中心还将为阿肯色州INBRE的所有研究人员提供位于主要机构的其他核心设施的访问，例如DNA测序和流式细胞术。此外，通过INBRE财团，阿肯色州INBRE将与其他INBRE建立网络，以访问阿肯色州目前可能没有的设施(参见http://louisville.edu/research/genomics/content/microarray_services.shtml和肯塔基州INBRE和路易斯维尔大学微阵列设施董事的支持信，肯塔基州路易斯维尔)。 科学研究核心还致力于帮助学生和教师参加暑期导师研究奖学金计划(请参阅外联核心)，使暑期研究员能够熟悉促进现代生物医学研究的技术，并最终更充分地参与INBRE计划。作为这一承诺的证据，非合伙人PUI的教员调查人员一直在使用现有的INBRE设施(见下文每个设施)。夏季举办的研讨会向全州的研究人员介绍了可用的、最先进的技术。 科学研究核心内的所有设施将根据需要与生物信息学核心互动，以满足阿肯色州INBRE合作伙伴PUI的需求(见目标，生物信息学核心)。目前，生物医学研究最令人望而生畏的方面之一是数据管理和生物信息学分析工具。对于蛋白质组学研究，蛋白质结构测定的数据收集需要新的信息技术(蛋白质信息学)来分析和解释数据。与生物信息学核心有关的教员将为数据分析提供必要的专业知识，包括对所有类型的数据进行更传统的统计分析，以及分析由微阵列或蛋白质组学技术产生的数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SCIENCE RESEARCH CORE The Arkansas INBRE proposes to continue its Science Research Core, which will comprise services offered by three scientific research facilities at two lead institutions. These core facilities will provide investigators throughout the state with access to the instrumentation and expertise needed for three key areas of modern biotechnologyproteomics, digital microscopy, and DNA damage and toxicology. These three areas were chosen because the technical expertise and, especially, the instrumentation of each facility are not commonly available, even in an average, well-funded research laboratory, particularly not at four-year PUIs. These three facilities were especially chosen to address the specific needs of the 13 Arkansas INBRE Project Leaders (see Table 11). With access to these technologies for specific studies, researchers can then pursue and extend research projects in their own laboratories. The use of these facilities should help researchers obtain necessary preliminary data to apply for extramural research funding. Arkansas INBRE support for the Science Research Core and its three facilities is primarily intended to guarantee access to these facilities by PUI investigators (both faculty and students). This support is not sufficient to completely sustain these facilities, which derive additional, more substantial support from other grant funding sources and from the institutions at which they are located (UAMS and UAF). The three facilities, described in detail below, have the goal of providing expertise and equipment for the following purposes: + Proteomics: To enable researchers to study protein structure and function using state-of-the-art instrumentation; + Digital Microscopy: To allow researchers to locate macromolecules within cells and tissues, thereby better assessing their function in normal and diseased states; and + DNA Damage and Toxicology: To allow researchers to incorporate DNA damage and toxicity analyses (as in cell injury and death) into their studies of normal and diseased cells and tissues. Each Science Research Core facility will also be committed to educating and training Arkansas INBRE faculty and students at both partner and non-partner PUIs, in sophisticated technologies specific to each facility. Hence, all facilities will provide workshops or demonstration sessions relevant to the instrumentation and expertise available. These offerings will be organized by leaders of all Science Research Core facilities and publicized by the Administrative Core statewide. The Science Research Core will also provide access to other Core Facilities located at the lead institutions, for example, DNA Sequencing and Flow Cytometry, for all researchers within the Arkansas INBRE. In addition, through the INBRE consortium, the Arkansas INBRE will network with other INBREs to obtain access to facilities that may not currently be available in Arkansas (see http://louisville.edu/research/genomics/content/microarray_services.shtml and letter of support from the directors of the Kentucky INBRE and the University of Louisville Microarray Facility, Louisville, KY). The Science Research Core is also committed to assisting both student and faculty participants in the Summer Mentored Research Fellowship Program (see Outreach Core), enabling summer fellows to become familiar with technologies that facilitate modern biomedical research and, ultimately, to participate more fully in the INBRE program. As evidence of this commitment, faculty investigators at non-partner PUIs have been using the current INBRE facilities (see below under each facility). Workshops offered during the summer introduce researchers from across the state to available, state-of-the-art technologies. All facilities within the Science Research Core will interact as needed with the Bioinformatics Core to provide for the needs of the Arkansas INBRE partner PUIs (see Objectives, Bioinformatics Core). At present, one of the most daunting aspects of biomedical research concerns data management and bioinformatics tools for analysis. For proteomics research, data collection for protein structure determination requires new information technologies (protein informatics) to analyze and interpret the data. Faculty associated with the Bioinformatics Core will be available to provide the requisite expertise for data analysis, including more conventional statistical analysis of all types of data and analysis of data generated by microarray or proteomics techniques.