PILOT PROJECTS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 解释感染因子的进化变化是21世纪世纪医学的一个关键挑战。影响人类健康的病原体迅速演变的例子比比皆是。特别相关的是抗生素耐药性的演变， 包括细菌、真菌和病毒在内的各种病原体中的“超级细菌”，以及与病原体入侵新宿主相关的进化。在这里，爱达荷州大学COBRE赠款的两个新项目，旨在促进过渡到 通过支持两位杰出的年轻研究人员进行生物医学相关的研究，以加速他们向独立资助的过渡。第一个项目将研究 细菌生物膜中抗生素抗性的演变，众所周知它们对抗生素处理的抗性，具有两个特定目的：（1）比较在生物膜中生长的细菌与分批培养中生长的细菌中演变的抗生素抗性的程度，和（2）通过将研究扩展到其他物种和生长条件来评估这些发现的一般性。这些目标将通过在实验室中培养细菌生物膜并测量它们的表型 随着时间而改变第二个项目开发一个模型系统，用于研究脊椎动物中新出现的真菌病原体。 它的重点是获得一个最近发现的真菌病原体，壶菌Batrachochytrium的进化机理的理解 Dendrobaustus（Bd）是导致世界各地两栖动物数量下降的原因。 有两个具体目标：（1）评估与向致病性转变相关的基因组变化，以及（2）评估与超强毒力相关的遗传特征。这些目标将通过对非Bd壶菌和毒力不同的Bd菌株的全基因组进行测序来实现，然后使用比较和功能基因组学方法来鉴定候选致病性/毒力基因。这一补充将加速 在爱达荷州的大学生物医学研究计划的发展，通过提供这些教师与足够的资源，以快速获取证明的原则数据，以支持全面的，独立的建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accounting for evolutionary change in infectious agents represents a key challenge for medicine in the 21st century. Examples of rapid evolution of pathogens affecting human health abound. Particularly relevant is the evolution of antibiotic resistance leading to "superbugs" in diverse pathogens, including bacteria, fungi, and viruses, and the evolution associated with the invasion of a pathgen onto a new host. Here, two new projects for the University of Idaho COBRE grant are proposed aimed at facilitating the transition into biomedically-relevant research by two outstanding young investigators by supporting them at a level that will accelerate their transition to independent funding. The first project will study the evolution of antibiotic resistance in bacterial biofilms, well-known for their resistance to antibiotic treatment, with two specific aims: (1) to compare the extent of evolved antibiotic resistance in bacteria grown in biofilms versus batch culture, and (2) to assess the generality of these findings by extending the study to other species and growth conditions. These aims will be addressed by growing bacterial biofilms in the lab and measuring how their phenotypes change through time. The second project develops a model system for studies of emerging fungal pathogens in vertebrates. It focuses on gaining a mechanistic understading of the evolution of a recently discovered fungal pathogen, the chytrid Batrachochytrium dendrobatidis (Bd), responsible for amphibian population declines around the world. there are two specific aims: (1) to evaluate the genomic changes associated with the transition to pathogenicity and (2) to evaluate the genetic signatures associated with hyper-virulence. These aims will be achieved by sequencing whole genomes of non-Bd chytrids and Bd strains that differ in virulence, then using comparative and functional genomics approaches to identify candidate pathogenicity/virulence genes. This supplement will accelerate the development of biomedical research programs at the University of Idaho by providing these faculty with sufficient resources to rapidly acquire proof-of-principle data to support full-scale, independent proposals.