Rapid breath detection of colorectal cancer with a colorimetric sensor array

描述(由申请人提供)：项目摘要/摘要最近有报道称，狗可以从呼出的气味中检测出结肠癌的存在，具有91%的选择性和99%的特异性(Sonoda，H.等人，Gut，2011，60,814)。一种可以进行类似测试的设备的前景开启了一种快速、非侵入性、准确和廉价的结肠癌呼气测试的前景。ISense Medical Corp.(IMC)传感器的工作原理类似于生物嗅觉，因为呼吸样本激活了多种交叉反应的化学传感器阵列。先前的工作已经表明，这种传感器系统基于嵌入在非常高表面积的纳米孔溶胶-凝胶基质中的比色指示剂，能够区分复杂的混合物(Suslick，B.等人，分析。化学。2010年、82年、2067年)，最近在克利夫兰临床基金会(CCF)的临床测试中显示，从呼气中跨阶段检测肺癌的存在，准确率为85%(Mazzone，P.等人，J.Thoracic Oncology，印刷中)。根据这份合同，我们建议定制纳米孔指示器阵列，以优化其对活动性结肠癌小分子代谢物的敏感度，并在第二阶段在CCF进行一项试点研究，以量化从呼吸到结肠癌分期检测的准确性。 公共卫生相关性：项目简介拟议的研究工作将开发一种快速、廉价和非侵入性的结肠癌诊断测试，作为后续结肠镜检查的提示。这项技术的进一步应用将是一种呼吸测试，以非侵入性和廉价的方式监测化疗的疗效。

DESCRIPTION (provided by applicant): Project Summary/Abstract Recently it has been reported that canines can detect the presence of colon cancer from the scent of exhaled breath, with 91% selectivity and 99% specificity (Sonoda, H. et al., Gut, 2011, 60, 814). The prospect of a device which can perform similarly opens the prospect of a rapid, non-invasive, accurate and inexpensive breath test for colon cancer. The iSense Medical Corp. (IMC) sensor operates analogously to biological olfaction in that a diverse array of cross- reactive chemical sensors is activated by the breath sample. Prior work has shown this sensor system, based on colorimetric indicators embedded in a nanoporous sol-gel matrix of very high surface area, to be capable of discrimination of complex mixtures (Suslick, B. et al., Anal. Chem. 2010, 82, 2067), and has recently been shown in clinical testing at the Cleveland Clinic Foundation (CCF) to detect the presence of lung cancer from exhaled breath, across stages, with accuracy of 85% (Mazzone, P. et al., J. Thoracic Oncology, in press). Under this contract we propose to customize the nanoporous indicator array to optimize its sensitivity to small molecule metabolites of active colon tumor, and in Phase 2 conduct a pilot study at CCF to quantify accuracy of detection of colon cancer from breath, by stage. PUBLIC HEALTH RELEVANCE: Project Narrative The proposed research effort will develop a rapid, inexpensive and non-invasive diagnostic test for the presence of colon cancer, as a prompt for subsequent colonoscopic examination. A further application of this technology would be a breath test to non-invasively and inexpensively monitor the efficacy of chemotherapy treatment.