Small Animal Core: Models of Acute Lung Injury

小动物核心：急性肺损伤（ALI）模型将提供两个主要核心功能， PPG的研究人员和五个研究项目。第一个功能是提供小鼠模型， ALI（LPS诱导和眩晕诱导），核心确保具有完整范围的可重复研究， 专业知识和工具，以获得有关内皮细胞和分子基础的关键信息， 屏障渗透性核心，使用已发表的技术和参数的急性肺损伤，将 采用最先进的肺部病理生理学评价、干预、数据分析， 解释，以便深入了解临床相关管理的疗效和机制 方法和促进基础研究向临床竞技场的转化。核心将采取 利用芝加哥大学小动物成像中心（包括 MRI，CT光学成像等。该核心提供渗透性，炎症， 水肿、血管渗漏和生物标志物。第二个核心功能是照顾野生型和基因工程 小鼠，并在指定的情况下产生新的转基因小鼠。核心 领导人聚集了有才华的和出版的人才，并享受新建的实验室设施， 满足广泛的实验生理和成像需求。核心C人员具备专业 经验跨越生理学，显微外科，生物学和动物护理领域，并已 提供了与所有五个研究项目的研究人员进行连贯互动的令人信服的证据 提供与我们的PPG项目相关的关键初步成果。

The Small Animal Core: Models of Acute Lung Injury (ALI) will provide two major core functions to support the PPG's research investigators and five research projects. The first function is providing murine models of ALI (LPS- and vertilator-induced) with the core ensuring reproducible studies with a complete range of expertise and tools to obtain critical information pertaining to the cellular and molecular basis of endothelial barrier permeability. The Core, using well published techniques and parameters of acute lung injury, will employ state-of-the-art evaluation of pulmonary pathophysiology, interventions, data analysis, and interpretation, in order to provide insight into the efficacy and mechanisms of clinically relevant management approaches and to facilitate the translation of basic research to the clinical arena. The Core will take advantage of the resources available at the University of Chicago Small Animal Imaging core (which includes MRI, CT Optical Imaging, etc. This Core provides quantitative measurements of permeability, inflammation, edema, vascular leak and biomarkers. A second core function is to care for the wild type and geneticallyengineered mice utilized in this Program and to generate novel transgenic mice where indicated. Core leaders have assembled talented and well published personnel and enjoy newly built laboratory facilities to satisfy a wide range of experimental physiological and imaging needs. Core C personnel have professional experience spanning the fields of physiology, microsurgery, biology, and animal care and has already provided convincing evidence of coherent interactions with investigators of all five research projects delivering key preliminary results related to our PPG projects.