X-Ray Chrystallography

X射线晶体学共享资源 X射线晶体学共享资源为KCC研究人员提供了获得结构 与他们的研究兴趣直接相关的信息。这包括从头结构的确定， 点突变的结构后果，与包括抑制剂在内的小分子的共结晶， 潜在的治疗剂和多组分系统，包括蛋白质、DNA和/或RNA。虽然结构 决定是一个高度专业化的，劳动密集型的奋进，从这些获得的信息 研究经常解决看似完全不同的生物化学数据，并总是改善未来 研究工作。 在改善KCC社区使用该设施的机会方面取得了重大进展。例如，一个 液体处理机器人现在以显著更少的材料和个人努力来测试结晶可行性， 使得初步筛选实验具有成本效益。这鼓励了一些KCC成员， 考虑在他们的项目中使用结构生物学。生物化学与分子生物学系 认识到结构生物学对大学的重要性，并在结构生物学方面增加了一个职位。 助理教授级别的生物学。随着用户和新员工的增加，我们预计 许多新的结构即将出现，大多数与癌症的分子起源直接相关。

X-RAY CRYSTALLOGRAPHY SHARED RESOURCE The X-ray Crystallography shared resource provides KCC investigators the ability to obtain structural information directly relevant to their research interests. This includes the determination of de novo structures, structural consequences of point mutations, co-crystallization with small molecules including inhibitors and potential therapeutics, and multicomponent systems including proteins, DNA and/or RNA. Although structure determination is a highly specialized, labor-intensive endeavor, the information obtained from these investigations frequently addresses seemingly disparate biochemical data and invariably improves future research efforts. Significant progress has been made to improve access to the facility for the KCC community. For instance, a liquid handling robot now tests for crystallization feasibility with significantly less material and personal effort, making preliminary screening experiments cost effective. This has encouraged a number of KCC members to consider employing structural biology in their program. The Department of Biochemistry and Molecular Biology recognizes the significance of structural biology to the university, and has made an additional hire in structural biology at the assistant professor level. With the increase of users and the new hire, we anticipate that a number of new structures will be forthcoming, most with immediate relevance to molecular origin of cancer.