Acquisition of a Transmission Electron Microscope

描述（由申请人提供）：位于博尔德科罗拉多大学分子、细胞和发育生物学部门的MCDB电子显微镜服务部正在寻求资金，从FEI公司购买FEI Tecnai G2 Spirit生物孪生透射电子显微镜（TEM）。需要一台新的TEM来取代我们已有23年历史的飞利浦CM10 TEM，这台性能不佳的显微镜越来越难以维护，目前正在为MCDB EM服务的用户服务。FEI G2 Spirit Bio Twin电子显微镜是一款非常通用的120 KV TEM，适用于大多数电子显微镜（EM）在细胞和分子显微镜中的应用。它的高对比度镜头非常适合于广泛的标本，是理想的低对比度生物标本，如负染色细胞制剂，高分辨率冷冻断裂复制品和传统的薄塑料切片。Tecnai G2 Spirit BioTwin配备了一个计算机控制的+/- 70度可倾斜测角仪平台，可以容纳传统的标本支架，用于控制倾斜轴的旋转支架，用于电子断层扫描（三维成像）的高倾斜双轴支架和用于成像玻璃化标本的最先进的LN2冷冻支架。7位美国国立卫生研究院资助的主要用户正在提交该申请：Bruce Eaton博士和Daniel Feldheim博士（生物金属纳米颗粒的合作），Robert Garcea博士（病毒结构和组装），Andreas Hoenger博士（微管结合蛋白结构，技术开发），Leslie Leinwand博士（肌球蛋白突变和心脏病），Greg Odorizzi博士（核内体组装和结构），Michael Stowell博士（膜蛋白结构）和Mark Winey博士（MTOCs的结构和组装）。这台显微镜还将为科罗拉多大学和其他机构的一些未成年用户提供服务。所有用户都需要5埃的点对点分辨率，这台显微镜和要求的Eagle 2K x 2K数码相机系统。他们的许多项目依赖于该仪器的平台和所需的标本支架提供的方便的标本倾斜轴控制。总的来说，主要和次要的用户研究各种各样的细胞生物学主题或分子结构，这些主题与病毒学，癌症，心脏病，衰老，

DESCRIPTION (provided by applicant): The MCDB Electron Microscopy Service, located in the Department of Molecular, Cellular and Developmental Biology at the University of Colorado in Boulder, seeks funding to purchase a FEI Tecnai G2 Spirit Bio Twin Transmission Electron Microscope (TEM) from the FEI Company. A new TEM is needed to replace our 23-year-old Philips CM10 TEM, an underperforming microscope that is increasingly difficult to maintain and is currently serving the users of the MCDB EM Service. The FEI G2 Spirit Bio Twin electron microscope is a very versatile 120 KV TEM that is appropriate for most electron microscopy (EM) applications in cellular and molecular microscopy. Its high-contrast lens is well suited to a wide range of specimens and is ideal for low-contrast biological specimens such as negatively stained cellular preparations, high- resolution freeze-fracture replicas and conventional thin plastic sections. The Tecnai G2 Spirit BioTwin is equipped with a computer controlled +/- 70 degree tiltable goniometer stage that can accommodate conventional specimen holders, rotating holders for control of the tilt axis, high- tilt-dual-axis holders for electron tomography(3-dimensional imaging) and state-of-the-art LN2 cryo-holders for imaging vitrified specimens. Seven NIH-funded major users are submitting this application: Dr. Bruce Eaton and Dr. Daniel Feldheim (collaboration on bio-metalic nanoparticles), Dr. Robert Garcea (virus structure and assembly), Dr. Andreas Hoenger (microtubule binding protein structure, technology development), Dr. Leslie Leinwand (myosin mutations and heart disease), Dr. Greg Odorizzi (endosome assembly and structure), Dr. Michael Stowell (membrane protein structure), and Dr. Mark Winey (structure and assembly of MTOCs). The microscope will also service a collection of minor users, both from the University of Colorado and from other institutions. All of the users require the 5-Angstrom point-to-point resolution obtainable with this microscope and the requested Eagle 2K x 2K digital camera system. Many of their projects depend upon the convenient control of the specimen tilt axis provided by this instrument's stage and the requested specimen holders. Altogether, the major and minor users study a wide variety of cell biology topics or molecular structures that are relevant to virology, cancer, heart disease, aging, neurodegenerative diseases, and diagnostic and therapeutic strategies.