Olympus FV1000MPE Multiphoton Microscope

描述（由申请人提供）：本申请的目的是申请资金购买Olympus FV 1000 MPE多光子显微镜。本申请中概述的系统被配置为专用多光子荧光深层组织成像系统。该系统内置的各种功能被设计为在具有多种应用的大量使用的成像核心设施中具有高度通用性。我们专门定制了一个专用系统，以在目前不适合我们的共聚焦显微镜的穿透深度进行成像，并通过二次谐波非线性光学显微镜技术可视化未染色的大分子结构，如胶原蛋白网络。购买这一重要设备将直接加强九个NIH资助的“主要用户”的工作。FV 1000 MPE在填补西北大学体内成像的迫切空白方面至关重要，并将使联邦资助的研究人员能够检查重要的生物过程，如新生血管形成，伤口修复，神经元内运输，基质重塑和肿瘤侵袭。我们已经使用与NIH资助的工作直接相关的生物/动物标本对该仪器进行了现场测试。我们在这里提出的初步数据，为每一个应用程序，我们建议执行的系统。提案中提供的初步结果包括目前正在提交出版的几个高影响力数据，强调了该仪器的科学影响和适用性，以及我们的技术专业知识。在为期9周的仪器演示期间，该仪器被满负荷使用，强调了对Olympus FV 1000 MPE的迫切需求。 公共卫生相关性：FV 1000 MPE将加强许多基础和转化研究项目。该系统将安装在一个设施，迎合~150个实验室，并将fulill在西北深层组织成像的迫切需要。

DESCRIPTION (provided by applicant): The objective of this application is to request funds to purchase the Olympus FV1000MPE multiphoton microscope. The system as outlined in this request is configured to be a dedicated multiphoton fluorescence deep-tissue imaging system. The various features built into the system are designed to be highly versatile in a heavily used imaging core facility with a multitude of applications. We have specifically tailored a dedicated system to perform imaging at penetration depth currently not suitable for our confocal microscopes and to visualize unstained macromolecular structures such as collagen network through second harmonic wave non-linear optical microscopy technique. The purchase of this important equipment will directly enhance the work of nine NIH-funded "major users". The FV1000MPE is crucial in filling an urgent gap in in vivo imaging at Northwestern University and will allow the federally funded investigators to examine important biological processes such as neovascularization, wound repair, intraneuronal transport, matrix remodeling and tumor invasion in the native microenvironment. We have tested this instrument on-site using biological/animal specimens directly related to the NIH-funded work. We present here preliminary data for each and every application we propose to perform on the system. The preliminary results presented in the proposal include several high impact data that are now being submitted for publication, highlighting the scientific impact and suitability of the instrument, as well as our technical expertise. During the 9-week instrument demo period, this instrument was used at full capacity, underscoring the urgent need of the Olympus FV1000MPE. PUBLIC HEALTH RELEVANCE: The FV1000MPE will enhance many basic and translational research projects. The system will be installed in a facility that caters to ~150 labs and will fulill an urgent need in deep tissue imaging at Northwestern.