Cell Imaging

现代癌症研究需要最先进的细胞和组织成像技术。这些技术 包括在光镜和电子显微镜水平上的免疫定位研究，使用 用荧光探针标记并通过转基因或显微注射引入的分子，以及先进的 图像记录和分析。先进的成像仪器，如电子显微镜、共焦 而多光子显微镜不仅非常昂贵，而且还需要技术援助才能达到最佳 结果。出于这些原因，至关重要的是拥有一个核心成像设施，以提供访问 仪器和技术专长。20多年前成立，作为一项部门服务，细胞 影像设施已演变为医学院的核心设施，得到了医务人员的支持 NU负责研究的副总裁，以及罗伯特·H·卢里综合癌症中心。这个 该机构的主任周登亮博士是光学显微镜成像方面的专家，特别是在 先进的活细胞荧光显微镜。还可以聘请全职电子显微镜技术专家 进行常规的细胞和组织包埋和切片，以及先进的技术，如免疫金 本地化，并在这些技术方面培训用户。该设施为数百名用户提供服务，这些用户来自 全校100多个研究小组。由于独特的专业知识和工作人员 致力于开发尖端成像应用，该设施已被众多 领先的制造商作为他们的仪器和软件的Beta测试点。更重要的是，该设施 已被尼康公认为三大国家卓越影像中心之一，因此受益 通过尼康赠送的几个最先进的设备，西北大学获得了巨大的成功。大约50 癌症中心成员使用了同行评议的资金，占总设施使用量的百分比。装备 包括两台透射式电子显微镜、两台共焦显微镜、一台旋转圆盘共焦显微镜 内反射荧光(TIRF)显微镜，三种先进的数字成像光学显微镜 系统和能力，包括微量注射、荧光、延时摄影、钙 成像、FRAP和FRET。辅助设备包括旋转式阴影蒸发器、缩微仪、彩色 打印机、数字扫描仪、胶片录像机、暗室、计算机和图像分析软件。

Modern cancer research requires state-of-the-art cell and tissue imaging technology. These techniques include immunolocalization studies at both the light and electron microscopic levels, live cell studies using molecules tagged with fluorescent probes and introduced by transfection or microinjection, and advanced image recording and analysis. Sophisticated imaging instruments such as electron microscopes, confocal and multi-photon microscopes are not only very expensive, but also require technical assistance for optimal results. For these reasons, it is essential to have a core imaging facility that provides access to both the instruments and technical expertise. Established more than 20 years ago as a departmental service, the Cell Imaging Facility has evolved into a Medical School Core Facility that receives support from the medical school, the Vice President for Research at NU, and the Robert H. Lurie Comprehensive Cancer Center. The Facility's Director, Dr. Teng-Leong Chew, Ph.D., is an expert in light microscopic imaging, especially in advanced live cell fluorescence microscopy. A full time electron microscope technologist is also available to perform routine cell and tissue embedding and sectioning as well as advanced techniques such as immunogold localization, and to train users in these techniques. The facility serves several hundred users from more than 100 research groups throughout the University. Due to the unique expertise as well as the staffs commitment to developing cutting-edge imaging application, the facility has been selected by numerous leading manufacturers as the beta test sites for their instruments and software. More importantly, the facility has been recognized as one of the three National Imaging Centers of Excellence by Nikon, thus benefiting Northwestern immensely through Nikon's gift of several state-of the-art equipments. Approximately 50 percent of total facility usage has been by Cancer Center members with peer reviewed funding. Equipment includes two transmission electron microscopes, two confocal microscopes, one spinning disc confocal, total internal reflection fluorescence (TIRF) microscope, three advanced light microscopes with digital imaging systems and capabilities that include microinjection, epifluoresence, time lapse photography, calcium imaging, FRAP, and FRET. Ancillary equipment includes rotary shadowing evaporators, microtomes, color printers, digital scanner, film recorder, dark room, and computers and software for image analysis.