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Zeiss PALM Laser Capture Microdissection System

蔡司 PALM 激光捕获显微切割系统

基本信息

批准号：
7594942
负责人：
Teng-Leong Chew
金额：
$ 25.15万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2010-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This application is to request funds to purchase the Zeiss PALM MicroBeam Laser Microdissection and Pressure Catapulting (LMPC) System. The system set-up proposed in this request is tailor-designed to be highly versatile in a heavily used imaging core facility with a multitude of applications and will take full advantage of the unique features afforded by the PALM microdissection system. The system will be (1) enclosed by a heated CO2 incubation chamber for long term live cell imaging and (2) will be equipped with Zeiss Apotome structured illumination grid for reasonably fast high resolution live cell imaging. (3) The system will also have two different digital to cater for the distinct imaging need for brightfield and low-light live cell imaging. This configuration thus sets this system apart from a regular microdissection system as it is perfectly suited to perform micro-harvesting of tissue specimens as well as the combinatorial application of subcellular microdissection and live cell microscopy. Specifically, this system is specifically configured to cater to the wide-ranging need encountered in this heavily used Cell Imaging Facility by meeting the demands of the following applications: (1) procurement of thick specimen, (2) micro-harvesting of archival tissue specimen, (3) live cell harvesting for subsequent recultivation, (4) micro-severing of cellular processes, (5) patterned wounding of live cell monolayer, and (6) microdissection of portion of nuclear membrane. Working closely with Carl Zeiss Inc., we have challenged the PALM system in our imaging facility with this remarkably wide spectrum of demanding application. We present here preliminary data for each and every application we propose to perform on the system, thus provide important evidence that this is indeed the system that will deliver all the instrumental benefits we anticipate and need. PUBLIC HEALTH RELEVANCE: This specially configured PALM Laser Microdissection System will enhance many basic and translational research projects. The installation of this versatile system in a core facility catering for ~300 labs will ensure that the maximum utilization of its full advantage.

描述（由申请人提供）：本申请旨在申请购买Zeiss PALM微束激光显微切割和压力催化（LMPC）系统的资金。本申请中提出的系统设置是专门设计的，在大量使用的成像核心设施中具有高度通用性，具有多种应用，并将充分利用PALM显微切割系统提供的独特功能。该系统将（1）被加热的CO2孵育室封闭，用于长期活细胞成像，（2）将配备Zeiss Apotome结构化照明网格，用于合理快速的高分辨率活细胞成像。(3)该系统还将有两个不同的数字，以满足明场和低光活细胞成像的不同成像需求。因此，这种配置将该系统与常规显微切割系统区分开来，因为它非常适合执行组织标本的显微采集以及亚细胞显微切割和活细胞显微镜的组合应用。具体而言，该系统经过专门配置，通过满足以下应用的需求，满足在这种大量使用的细胞成像设备中遇到的广泛需求：（1）厚标本的获取，（2）存档组织标本的微收获，（3）活细胞收获用于随后的再培养，（4）细胞突起的微切割，（5）活细胞单层的图案化创伤，（6）部分核膜的显微切割。与卡尔蔡司公司密切合作，我们已经在成像设备中对PALM系统提出了挑战，其具有非常广泛的要求应用。我们在这里提出的每个应用程序的初步数据，我们建议执行的系统，从而提供了重要的证据，这确实是系统，将提供所有的工具，我们预期和需要的好处。公共卫生相关性：这种特殊配置的PALM激光显微切割系统将增强许多基础和转化研究项目。在一个可容纳约300个实验室的核心设施中安装这个多功能系统将确保最大限度地利用其全部优势。