Cell Imaging

细胞成像

• 批准号: 8486517
• 负责人: Teng-Leong Chew
• 金额: $ 18.39万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-06 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8486517
• 关键词: Calcium; Cancer Center; Cells; Color; Comprehensive Cancer Center; Computer software; Core Facility; Doctor of Philosophy; Electron Microscope; Electrons; Equipment; Film; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Funding; Gifts; Image; Image Analysis; Imaging technology; Life; Light; Light Microscope; Manufacturer Name; Microinjections; Microscope; Microscopic; Microtome - medical device; Peer Review; Photography; Research; Services; Shadowing (Histology); Site; System; Technical Expertise; Techniques; Testing; Time; Tissue Embedding; Tissues; Training; Transfection; Universities; anticancer research; cellular imaging; digital; digital imaging; fluorescence microscope; instrument; medical schools; member; multi-photon; transmission process

Modern cancer research requires state-of-the-art cell and tissue imaging technology. These techniques include immunolocalization studies at both the light and electron microscopic levels, live cell studies using molecules tagged with fluorescent probes and introduced by transfection or microinjection, and advanced image recording and analysis. Sophisticated imaging instruments such as electron microscopes, confocal and multi-photon microscopes are not only very expensive, but also require technical assistance for optimal results. For these reasons, it is essential to have a core imaging facility that provides access to both the instruments and technical expertise. Established more than 20 years ago as a departmental service, the Cell Imaging Facility has evolved into a Medical School Core Facility that receives support from the medical school, the Vice President for Research at NU, and the Robert H. Lurie Comprehensive Cancer Center. The Facility's Director, Dr. Teng-Leong Chew, Ph.D., is an expert in light microscopic imaging, especially in advanced live cell fluorescence microscopy. A full time electron microscope technologist is also available to perform routine cell and tissue embedding and sectioning as well as advanced techniques such as immunogold localization, and to train users in these techniques. The facility serves several hundred users from more than 100 research groups throughout the University. Due to the unique expertise as well as the staffs commitment to developing cutting-edge imaging application, the facility has been selected by numerous leading manufacturers as the beta test sites for their instruments and software. More importantly, the facility has been recognized as one of the three National Imaging Centers of Excellence by Nikon, thus benefiting Northwestern immensely through Nikon's gift of several state-of the-art equipments. Approximately 50 percent of total facility usage has been by Cancer Center members with peer reviewed funding. Equipment includes two transmission electron microscopes, two confocal microscopes, one spinning disc confocal, total internal reflection fluorescence (TIRF) microscope, three advanced light microscopes with digital imaging systems and capabilities that include microinjection, epifluoresence, time lapse photography, calcium imaging, FRAP, and FRET. Ancillary equipment includes rotary shadowing evaporators, microtomes, color printers, digital scanner, film recorder, dark room, and computers and software for image analysis.

现代癌症研究需要最先进的细胞和组织成像技术。这些技术 包括光学和电子显微镜水平的免疫定位研究、使用 用荧光探针标记并通过转染或显微注射引入的分子， 图像记录和分析。先进的成像仪器，如电子显微镜，共聚焦显微镜， 多光子显微镜不仅非常昂贵，而且还需要技术援助， 结果由于这些原因，必须有一个核心成像设施， 工具和技术专长。该小组成立于20多年前，是一个部门服务， 成像设施已发展成为一个医学院的核心设施，接受来自医疗 学校，在NU研究副总裁，和罗伯特H。卢里综合癌症中心。的 中心主任，Dr. Teng-Leong Chew，Ph.D.，是光学显微镜成像的专家，尤其是在 先进的活细胞荧光显微镜。一个全职的电子显微镜技术专家也可提供， 进行常规的细胞和组织包埋和切片以及先进的技术，如免疫金 本地化，并在这些技术中培训用户。该设施为数百名用户提供服务， 在整个大学有100多个研究小组。由于独特的专业知识以及工作人员 致力于开发尖端的成像应用，该设施已被众多 领先的制造商作为其仪器和软件的beta测试站点。更重要的是， 已被尼康认定为三个国家成像卓越中心之一，从而受益于 西北极大地通过尼康的礼物的几个国家的最先进的设备。大约50 癌症中心的成员使用了全部设施的10%，并获得了同行评审的资金。设备 包括两个透射电子显微镜、两个共焦显微镜、一个共焦旋转圆盘， 内反射荧光（TIRF）显微镜，三个先进的光学显微镜与数字成像 系统和能力，包括显微注射，epifluorescence，时间推移摄影，钙 成像、FRAP和FRET。辅助设备包括旋转阴影蒸发器、切片机、彩色 打印机、数字扫描仪、胶片记录器、暗室以及用于图像分析的计算机和软件。