Administrative/Statistics/Imaging Core

核心A的管理部分为Schreiber博士提供了科学领导和谨慎的保护时间 本计划项目的指导。这种领导力对于实现该计划的目标至关重要。 该项目依赖于将具有高度互补性但根本不同的技能和 运营模式和非常多样化的方法和技术。有效的生产性整合将取决于 关于将相互作用的科学家的物理隔离的负面影响降至最低。这需要 来自Schreiber博士的专门时间，用于仔细规划和使用长期轻松交互的方法 距离。为协调本计划项目的科技活动，核心将(一) 每月组织一次指导委员会会议和研究进展会议，与 四个项目，统计员和核心主任，认真规划议程，包括分发 将讨论的数据、手稿和论文，(2)组织更全面的季度会议 所有参与该计划的科技人员，(三)准备、分发和维护电子 报告、方案和图像的文件并分发相关研究文章，(Iv)准备和更新 现有的细胞系、基因构建、单抗和载体数据库，以及(V)今天的监测 支付、支持和组织一名外部科学顾问和一名外部合作者的访问 每年。核心A的统计部分为我们的统计学家卡里森博士提供了一个受保护的时间。这 为研究人员提供了一位经验丰富的学术统计学家，他致力于这项研究的成功 计划项目。这一安排将大大改善我们获得生物统计学咨询的机会 拟议研究的规划、执行和分析阶段。A型岩芯的成象部分 P.Charles Lin博士的领导为研究人员提供了继续使用一流成像技术的途径 范德比尔特研究所使用窗腔室技术进行光学成像。迁徙与本土化 将荧光T细胞或荧光标记分子转移到大型成熟肿瘤 使用蔡司多光子激光通过肿瘤一侧的窗口进行监测 扫描显微镜LSM 510 Meta。新型META(蔡司)光谱探测器可解决 这些不同的荧光染料通过使用专门的线性光谱分解提供高效和准确的 软件。肿瘤微环境的显微成像将为我们提供真实的 在所提出的各种模型中，注射到小鼠体内的细胞或分子的时间事件和精确位置。

The Administrative Part of Core A provides Dr. Schreiber protected time for scientific leadership and careful guidance of the Program Project. This leadership will be essential for achieving the goals of the program project that depend on integrating scientists with highly complementary but fundamentally different skills and operation modes and very diverse methodology and technology. Effective productive integration will depend on minimizing negative effects of the physical separation of the interacting scientists. This requires dedicated time from Dr. Schreiber for careful planning and using methods of easy interaction over long distances. For coordinating the scientific and technical activities of this program project, the Core will (i) organize a monthly steering committee meeting and research-in-progress meeting with the leaders of the four projects, the statistician and the core directors with careful planning of the agenda including distribution of data, manuscripts and papers to be discussed, (ii) organizes a more comprehensive quarterly meeting of all scientific and technical personnel involved in the program, (iii) prepare, distribute and maintain electronic files of reports, protocols and images and distribute relevant research articles, (iv) prepare and update existing databases for cell lines, gene constructs, monoclonal antibodies and vectors, and (v) monitor day-today expenses, support and organize the visits of one external scientific advisor and one external collaborator per year. The Statistics Part of Core A, provides a protected time to our statistician Dr. Karrison. This provides researchers with a highly experienced scholarly statistician who is dedicated to the success of this program project. This arrangement will give us much improved access to biostatistical consultation during the planning and execution and analysis stages of the proposed studies. The Imaging Part of Core A under the leadership of Dr. P. Charles Lin provides researchers with continued access to the first rate imaging institute at Vanderbilt for optical imaging using window chamber technology. The migration and localization of the fluorescent T cells or fluorochrome-tagged molecules to large well-established tumors will be monitored through a window opening on one side of these tumors using the Zeiss multiphoton laser scanning microscope LSM 510 META. The new META (Zeiss) spectral detectors resolve emissions from these different fluorescent dyes efficiently and exactly by providing linear spectral unmixing using specialized software. The microscopic imaging of the tumor microenvironment will give us detailed information on real time events and precise locality of cells or molecules injected into mice in the various models proposed.