GENE-TARGETTED SNP DISCOVERY IN RHESUS MACAQUES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目最初的目的是通过对无关的中国恒河猴和印度恒河猴的3‘非编码区进行测序，确定10,000个基因区域的单核苷酸多态(SNPs)。将对汇编的SNP数据进行分析，以确定印度和中国人群中的SNPs和等位基因频率，并在公共数据库DBSNP和MonkeySNP中报告发现。在过去的一年里，我们调整了我们的SNP发现战略，将下一代测序技术(Illumina短读格式)包括在内。我们使用六只印度恒河猴和六只中国恒河猴的前额叶皮质的mRNA完成了转录组测序。Sanger重新测序用于验证候选SNPs，并通知筛选界限以确保数据稳健。到目前为止，在这项研究中检测到了100多万个SNP。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project originally aimed to identify single nucleotide polymorphisms (SNPs) in 10,000 gene regions, by sequencing the 3' UTR of unrelated Chinese and Indian rhesus macaques. The compiled SNP data will be analyzed to identify SNPs and allele frequencies in both Indian and Chinese populations and to report the findings in the public databases dbSNP and MonkeySNP. In the past year we adjusted our SNP discovery strategy to include next generation sequencing technologies (Illumina short-read formats). We completed transcriptome sequencing using mRNA from the prefrontal cortex of six Indian and six Chinese rhesus macaques. Sanger re-sequencing was used to validate the candidate SNPs and to inform filtering limits to insure robust data. Over 1 million SNPs were detected in this study thus far.