LENS LASER STRATEGIES FOR PRESBYOPIA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目的：观察老年恒河猴透镜激光损伤前后中枢神经刺激的反应能力。 研究结果： 目前，接受透镜激光治疗的猴子被圈养以进行长期观察。 先前，将电极植入2只老年恒河猴的Edinger-Westphal核中，以便可以中枢刺激调节。此外，使用4只年轻恒河猴作为测试对象来校准飞秒激光。 在确定透镜激光对屈光反应的影响之前完成基线测试。飞秒激光手术似乎在猴眼中耐受良好。然而，在5只接受激光治疗的猴子中，有2只观察到小的视网膜灼伤（大小为1-2 mm）。激光照射后24小时开始的飞秒激光手术不会影响使用Hartinger符合折射仪获得折射测量的能力。动物未表现出异常行为或疼痛或不适迹象。 对其余4只恒河猴每半年进行一次裂隙灯检查。所有动物都保持正常视力。目前，这些动物继续受到监测，未来的实验计划正在考虑中。 本研究使用WNPRC动物和病理学服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To determine the accommodative ability in older rhesus monkeys following central stimulation before and after lens lasering. RESULTS: Currently, monkeys that received laser treatment of the lens are being housed for long-term observations. Previously, an electrode was implanted into the Edinger- Westphal nucleus of each of 2 older rhesus monkeys so that accommodation could be stimulated centrally. In addition, 4 young rhesus monkeys were used as test subjects to calibrate the femtosecond laser. Baseline testing was completed prior to determination of the effects of lens lasering on the accommodative response. The femtosecond laser procedure appears to be well tolerated in the monkey eye. However, small retinal burns (1-2 mm in size) were noted in 2 out of 5 monkeys lasered. The ability to obtain refractive measurements with the Hartinger coincidence refractometer was not compromised by the femtosecond laser procedure beginning 24 hours after lasering. Animals did not exhibit abnormal behavior or evidence of pain or discomfort. Slit lamp examinations are performed bi-annually in the remaining four rhesus monkeys. All animals maintain normal vision. Presently the animals continue to be monitored and future experimental plans are being considered. This research used WNPRC Animal and Pathology Services.