Flow Cytometry and High Throughput Screening Shared Resource

UNM共享流式细胞术和化学生物学资源由位于 UNM癌症研究设施和研究孵化器大楼（RIB），5分钟步行距离 细胞计数和筛选部分由布鲁斯爱德华兹博士指导，由布兰迪经营 Crowder和Catherine Prudom博士分别流式细胞术和筛查实验室 细胞计数器和其他可供癌症中心研究人员使用的设备。的 开发了使用流式细胞仪进行高通量筛选的能力， 进入一个成熟的高通量筛选操作，称为UNM分子发现中心 它是NIH路线图资助的分子图书馆探针生产中心网络的一部分。联合主任 Tudor Oprea领导了小分子计算和信息学方法的合作努力 在RIB发现。报告期内癌症中心成员发表>70篇文章 与资源使用的关联，包括仅基于计算方法的那些。二十六 代表所有4个癌症中心研究项目的成员使用资源服务（占总使用量的80%）， 25项研究赠款。目前对资源使用者的收费处于低到中等范围， 全国范围内的利率和癌症中心的成员有资格获得20%的共同支付的所有利率。资源用户是 单独接受仪器和软件使用培训。新用户可以通过以下方式执行试验项目： 在检测开发阶段免除使用费。该资源提供细胞分析，细胞 分类和离线数据分析作为标准服务。它最近引入了高通量筛选， 化学信息学，允许资源用户常规访问发明的新型HyperCyt技术， 由布鲁斯爱德华兹和拉里Sklar，癌症中心副主任基本 Research.该设施的总体目标是：a）为研究人员提供支持服务; B）培训 例如与个别研究人员进行特别工作和安排专门领域的讲习班; c） 与癌症中心的研究人员和学员合作，特别是在新研究的发展方面 在小分子鉴定中的应用，d）通过开发高通量的 使用384和1536孔板的能力和应用，化合物文库的筛选， 多重微珠分析的实现和化学生物学的化学信息学支持 项目;以及e）通过设施图尔斯参观、说明性海报展示、年度开放日等方式进行宣传 并定期更新资源网站。

The UNM Shared Flow Cytometry and Chemical Biology Resource consists of laboratories located in the UNM Cancer Research Facility and The Research Incubator Building (RIB), 5 minutes walking distance The Cytometry and Screening components are directed by Bruce Edwards, Ph.D., and operated by Brandy Crowder and Catherine Prudom, Ph.D. respectively. The Cytometry and Screening Lab houses flow cytometers and other equipment that are available for use by researchers in the Cancer Center. The Resource developed the capability for high throughput screening with flow cytometers which has evolved into a full-fledged High Throughput Screening operation called the UNM Center for Molecular Discovery which is part of the NIH Roadmap funded Molecular Libraries Probe Production Center Network. Co-Director Tudor Oprea leads collaborative efforts in computational and informatics approaches to small molecule discovery housed in RIB. During the reporting period. Cancer Center members published >70 articles in association with resource usage including those based on computational approaches alone. Twenty-six members representing all 4 Cancer Center research programs used resource services (80% of total use) in conjunction with 25 research grants. Current charge backs to resource users are in the low to mid-range of nation-wide rates and Cancer Center members qualify for 20% co-pay on all rates. Resource users are individually trained in instrument and software use. New users are enabled to perform pilot projects through a waiver of use charges during the assay development phase. The Resource provides cell analysis, cell sorting, and offline data analysis as standard services. It recently introduced high throughput screening and cheminformatics, allowing resource users routine access to novel HyperCyt technology invented and developed at UNM by Bruce Edwards and Larry Sklar, the Cancer Center Associate Director of Basic Research. The overall goals of the facility are to provide: a) support services to research faculty; b) training such as ad hoc work with individual researchers and scheduled workshops in specialized areas; c) collaboration with Cancer Center investigators and trainees, particularly in the development of new research applications in small molecule identification, d) innovation through the development of high throughput capabilities and applications for use of 384 and 1536 well plates, screening of compound libraries, implementation of multiplexed bead-based analysis, and cheminformatics support for chemical biology projects; as well as e) dissemination through facility tours, descriptive poster displays, an annual open house and periodic updating of the Resource web site.