High-Throughput BioMEMS

描述(由申请人提供)：我们将开发用于高通量、高含量、基于细胞的筛选、小的稀有细胞组分的表型和操作以及流式细胞中1-100个细胞的多重表达分析的方法和并行流动仪器。我们将为蛋白质错误折叠疾病，特别是帕金森氏症(模式生物：酵母)等神经退行性疾病的机械和小分子研究提供组合规模的方法。并用于(罕见的)原代造血干细胞(小鼠)的高含量细胞术研究。 公共卫生相关性：该计划将为基于细胞的高通量、高含量筛查(HCS)开发新的仪器和方法。该计划解决了严重限制HCS的瓶颈，用于(I)扩大到药物发现，(Ii)处理高度异质的原代细胞类型的小样本；(Iii)寻找稀有细胞(例如，寻找用于癌症诊断的多能细胞)和(Iv)基于图像的分类/浓缩。

DESCRIPTION (provided by applicant): We will develop methods and parallel flow instrumentation for high-throughput, high- content, cell-based screening, for phenotyping and manipulation of small rare-cell fractions, and for multiplexed expression analysis from 1-100 cells in flow. We will provide means for combinatorial scaling of mechanistic and small-molecule studies of protein misfolding disease, particularly neurodegenerative diseases such as Parkinson's (model organism: yeast). And for high-content cytometry studies with (rare) primary hematopoietic stem cells (mouse). PUBLIC HEALTH RELEVANCE: This program would develop new instrumentation and methods for cell-based high- throughput, high-content screening (HCS). The program addresses bottlenecks that severely limit HCS for (i) scale-up to drug discovery, (ii) for handling of small samples of highly heterogeneous primary cell types; (iii) for finding rare cells (e.g. finding pluripotent cells for cancer diagnostics) and, (iv) image-based sorting/enrichment.