Light Microscopy Core

NHLBI光学显微镜核心设施(LMCF)已经运行了12年。LMCF由克里斯蒂安·A·库姆斯博士和丹妮拉·A·马利德博士两人以及分布在三个地点的九台显微镜组成。到目前为止，我们已经帮助研究人员发表了170多篇论文，并在过去的12年里帮助了几乎每个NHLBI-DIR中心和分支机构。在2011-2012年间，我们得到帮助发表了22篇论文(截至本报告之时)。在LMCF进行的研究涉及许多疾病状态、基本细胞生物学以及新成像技术的开发和实施。LMCF的总体构成反映了NHLBI-DIR研究人员不断发展的光学显微镜需求，并承诺为NHLBI任务提供最大程度的援助。 该设施的任务是提供最先进的设备、培训和图像处理能力，以帮助NHLBI-DIR研究人员进行涉及光学显微镜的实验。在这个设施工作的研究人员可以期待核心人员提供适合他们研究的任何级别的支持。这可以包括先进的显微技术，如活体内双光子显微镜和超分辨率显微镜，到使用彩色相机进行更普通的广域成像。我们的重点是培训用户自己进行实验，尽管我们可以进行合作和各种形式的协助(实验计划、数据分析和图像处理等)。在需要的地方。 在这个核心已经存在的12年中，我们努力提供一种灵活且易于使用的设备，以满足研究人员的需求，并允许他们以高效的方式进行实验，即使他们之前没有显微镜经验。这些目标可以通过几种方式实现。首先，我们有一系列显微镜，可以提供广泛的显微镜技术。这些显微镜和现有的显微镜技术是根据研究所研究人员的具体需求而选择和开发的。除了显微镜，我们还提供全套图像处理程序和专用的位图像处理工作站。在这些程序缺乏图像处理能力的情况下，我们要么编写自己的图像处理程序。最后，我们提供了一个关于核心和显微教学(https://intramural.nhlbi.nih.gov/Cores/LMF/Pages/default.aspx).各个方面的综合网站我们与该网站合作的目标是让研究人员有一个一站式的地方来了解核心以及如何计划他们的实验。

The NHLBI Light Microscopy Core Facility (LMCF) facility has been in operation for twelve years. The LMCF consists of two people, Dr. Christian A. Combs and Dr. Daniela A. Malide, and nine microscopes located in three locations. To date we have helped researchers publish more than 170 papers and assisted almost every NHLBI-DIR Center and Branch in the past twelve years. In 2011-2012 we have help to publish twenty two papers (at the time of this report). Research conducted in the LMCF has been on many disease states, basic cell biology, and on development and implementation of new imaging techniques. The general makeup of the LMCF reflects the evolving light microscopy needs of NHLBI-DIR researchers and a commitment to provide a maximal level of assistance to the NHLBI mission. The mission of this facility is to provide state-of-the-art equipment, training, and image processing capabilities to assist NHLBI-DIR researchers in experiments involving light microscopy. Researchers that work in this facility can expect support from core personnel to whatever level suits their research. This can include advanced microscopy techniques like intra-vital two-photon microscopy and super-resolution microscopy to more ordinary wide-field imaging with a color camera Our emphasis is on training users to conduct the experiments themselves, although we are available for collaboration and all manner of assistance (experimental planning, data analysis and image processing, etc.) where required. Over the twelve years this core has been in existence we have endeavored to provide a flexible and easy to use facility that meets researchers needs and allows them to conduct their experiments in an efficient manner even if they have had no prior microscopy experience. These goals are met in several ways. First, we have an array of microscopes that offer a wide-range of microscopy techniques. These microscopes and the microscopy techniques available have been chosen and developed in response to the specific needs of researchers in the institute. In addition to the microscopes, we provide a full suite of image processing programs a dedicated 64-bit image processing workstation. Where image-processing capabilities are lacking in these programs we either write our own image processing programs. Last, we provide a comprehensive web site about all facets of the core and microscopy instruction (https://intramural.nhlbi.nih.gov/Cores/LMF/Pages/default.aspx). The goal we are working towards with the website is to allow researchers a one-stop place to learn about the core and how to plan their experiments.