CMPB Central Pathology Support

在过去的一年里，协作和支持导向的工作对研究产生了影响，并对一系列疾病和疾病过程的理解产生了影响，如心血管疾病（动脉粥样硬化），炎症性呼吸道疾病，肥胖和II型糖尿病，炎症和免疫介导的肾脏疾病，基因表达和基因调控，中轴骨骼发育，癌症和饮食限制的跨代影响。此外，还检查了雄性大鼠亚慢性研究中慢性进展性肾病加重与后续2年研究中肾肿瘤发生率增加之间的相关性。此外，小鼠表型分析小组还协助了一些成年基因工程小鼠项目的表型分析。 虽然该机构提供的服务重点是胚胎致死表型的组织学评估，但还提供了其他服务，以协助特殊尸检技术和RNA分离的特殊应用。激光显微切割小组与研究人员合作，建立了成功的协议，用于分离蛋白质（蛋白质印迹和蛋白质组学分析），RNA从冷冻和最近从激光显微切割，石蜡包埋组织（定量RT-PCR）和DNA（胚胎基因分型）从激光显微切割组织样本。免疫组化小组与研究人员合作，为各种独特的组织标记物建立免疫染色方案。

Collaborative and support oriented work has over the past year had an impact in the study, and on the understanding, of a spectrum of diseases and disease processes such as cardiovascular disease (atherosclerosis), inflammatory respiratory disease, obesity and type II diabetes, inflammatory and immune-mediated renal disease, gene expression and gene regulation, axial skeletal development, cancer and transgenerational effects of diet restriction. Additionally, associations between exacerbation of chronic progressive nephropathy in subchronic studies and increased incidence of renal tumors in subsequent 2-year studies was examined for male rats. In addition, the mouse phenotyping group has assisted with the phenotyping a few adult genetically engineered mice projects. While the focus of services provided by this facility has been on the histological assessment with embryonic lethal phenotypes, additional services have been provided in assisting with special necropsy techniques and special applications with regards to RNA isolation. The laser microdissection group has worked with investigators to establish successful protocols for the isolation of protein (western blots and proteomic analysis), RNA from frozen and more recently from laser-microdissected, paraffin-embedded tissue (quantitative RT-PCR) and DNA (embryo genotyping) from laser-microdissected tissue samples. The immunohistochemistry group has worked with investigators to establish immunostaining protocols for a variety of unique tissue markers.