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Protein Chemistry Core

Protein Chemistry Core
蛋白质化学核心
批准号：
8350140
负责人：
Deborah Morrison
金额：
$20.56万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/8350140
关键词：
AcetylationAmino Acid SequenceAmino AcidsBuffersCCRChargeCommunitiesComputer softwareDigestionDimensionsExtramural ActivitiesGelHigh Pressure Liquid ChromatographyImmunoblottingInstitutesIntramural ResearchIsoelectric FocusingLabelLaboratoriesLiquid substanceMapsMass Spectrum AnalysisMethodsMethylationModificationMolecular WeightN-terminalPeptide MappingPeptide Sequence DeterminationPeptidesPhasePhosphoamino AcidsPhosphorylationPhosphorylation SitePositioning AttributePost-Translational Protein ProcessingProcessProtein AnalysisProtein ChemistryProteinsProteomicsRadioactiveRadioactive SequencingResearch PersonnelSamplingSerineServicesSiteStaining methodStainsTechniquesTechnologyThreonineTwo-Dimensional Gel ElectrophoresisTyrosineUnited States National Institutes of Healthcomparativedrug modificationinterestpressureprotein aminoacid sequencetwo-dimensional

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中文摘要
翻译
在2010-2011年期间，蛋白质化学核心（PCC）为NCI-CCR以及其他NIH研究所的研究人员提供服务。此外，PCC与校外社区的实验室合作。对102份样品进行N-末端肽测序。处理65个样品用于HPLC肽分析。处理8个样品用于肽消化。对52份样品进行磷酸氨基酸分析。对45份样品进行2D凝胶电泳。将42个2D-凝胶染色或转移到immobilon用于进一步分析。
英文摘要
During 2010-2011, the Protein Chemistry Core (PCC) provided services to investigators in the NCI-CCR as well as other NIH institutes. In addition, the PCC collaborated with laboratories in the extramural community. 102 samples were processed for N-terminal peptide sequencing. 65 samples were processed for HPLC peptide analysis. 8 samples were processed for peptide digestion. 52 samples were processed for phosphoamino acid analysis. 45 samples were processed for 2D gel electrophoresis. 42 2D-gels were stained or transferred to immobilon for further analysis.
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Regulation of Ras-Dependent Signal Transduction Pathways
  • 批准号：
    8937711
  • 项目类别：
  • 资助金额：
    $60.81万
  • 财政年份：
    --
  • 负责人：
    Deborah Morrison
  • 依托单位：
    DIVISION OF BASIC SCIENCES - NCI
Role of Protein Scaffolds in RTK-Ras-dependent Signal Transduction
  • 批准号：
    9343799
  • 项目类别：
  • 资助金额：
    $60.29万
  • 财政年份：
    --
  • 负责人：
    Deborah Morrison
  • 依托单位：
    DIVISION OF BASIC SCIENCES - NCI
Role of Protein Scaffolds in RTKRas-dependent Signal Transduction
  • 批准号：
    9153776
  • 项目类别：
  • 资助金额：
    $66.94万
  • 财政年份：
    --
  • 负责人：
    Deborah Morrison
  • 依托单位：
    DIVISION OF BASIC SCIENCES - NCI
Regulation of Ras-Dependent Signal Transduction Pathways
  • 批准号：
    8552667
  • 项目类别：
  • 资助金额：
    $64.31万
  • 财政年份：
    --
  • 负责人：
    Deborah Morrison
  • 依托单位：
    DIVISION OF BASIC SCIENCES - NCI
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