SD COBRE: CELL IMAGING CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 成像核心的主要使命是促进桑福德研究/美国大学及其附属研究机构的显微成像需求。成像核心利用光和共聚焦显微镜来观察活细胞和固定细胞。目前，核心配备了三个共焦显微镜(尼康A1与TIRF，奥林巴斯FV1000和奥林巴斯FV300)，Imaris图像分析软件，和多个光学显微镜。成像核心协助研究人员进行免疫荧光染色、标记荧光蛋白可视化和三维图像重建。 通过为Cobre和CHRC实验室提供成像帮助，并通过将所有显微镜集中在我们机构内，我们在提高生产率的同时减少了成本和时间。成像和图像分析对于科布雷上的大多数项目都是必不可少的，成像核心使每个研究人员都可以获得这些关键分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary mission of the Imaging Core is to facilitate the microscopic imaging needs of Sanford Research/USD and our affiliated research institutions. The Imaging Core utilizes light and confocal microscopy to visualize both living and fixed cells. Currently the core is equipped with three confocal microscopes (Nikon A1 with TIRF, Olympus FV1000 and Olympus FV300), Imaris image analysis software, and multiple light microscopes. The Imaging Core assists researchers with immuno-fluorescent staining, tagged fluorescent protein visualization, and 3-dimensional image reconstruction. By providing imaging assistance to COBRE and CHRC labs, and by centralizing all microscopes within our institution, we reduce cost and time while increasing productivity. Imaging and image analysis is essential to most projects on the COBRE, and the Imaging Core makes these critical analyses available to every investigator.