MOLECULAR BIOLOGY/VIRAL VECTOR CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 分子生物学核心设施(MBCF)是在COBREII供资期间由另外两个核心，即病毒载体和微阵列核心合并而成的。它为用户提供了广泛技术方面的设备和专业知识，包括基因表达分析、基因分型、克隆、病毒载体生产和基础分子生物学。该设施目前提供的仪器和设备包括： +安捷伦(Stratagene)Mx3005P实时聚合酶链式反应分析仪 +安捷伦2100生物分析仪 +Axon GenePix 4000B微阵列扫描仪 +NanoDrop 1000D分光光度计 +MJ Research PTC-100热循环机 +Eppendorf 5810R冷藏离心机 +Forma Science-80C冷冻机 +罗氏通用探针库用于RT-PCR +Ariadne基因组学路径工作室软件 MBCF由David Poulsen博士执导，Corbin Schwanke担任工作人员。在过去的一年里，核心的资金完全由CEHS Cobre为环境健康科学中心提供的赠款提供。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular Biology Core Facility (MBCF) was formed during the COBREII funding period from the consolidation of two other cores, the Viral Vector and Microarray cores. It has provided users with equipment and expertise in a wide range of techniques, including gene expression analysis, genotyping, cloning, viral vector production, and basic molecular biology. Instruments and equipment currently available in the facility include: + Agilent (Stratagene) Mx3005p Real-time PCR Instrument + Agilent 2100 Bioanalyzer + Axon GenePix 4000B Microarray Scanner + NanoDrop 1000D Spectrophotometer + MJ Research PTC-100 Thermal Cycler + Eppendorf 5810R Refrigerated Centrifuge + Forma Scientific -80¿C Freezer + Roche Universal ProbeLibrary for RT-PCR + Ariadne Genomics Pathway Studio Software The MBCF has been directed by Dr. David Poulsen, and staffed by Corbin Schwanke. Over the past year funding for the core has been solely provided by the CEHS COBRE grants for the Center for Environmental Health Sciences.