ANTI-INFLAMMATORY EFFECTS OF PARASYMPATHETIC DRUGS ON ENDOMETRIAL CELLS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 子宫内膜异位症是一种炎症性疾病，子宫内膜异位症外植体的细胞具有侵袭性。炎症反应和侵袭反应都可以通过处理培养的子宫内膜间质细胞来模拟。本项目将研究副交感神经系统介体对培养的子宫内膜间质细胞的抗炎作用。实验室已经确定了THESC细胞中表达的炎症基因的特征，以回应巨噬细胞条件培养液的处理。在没有M受体激动剂匹罗卡品存在的情况下，将用巨噬细胞条件培养液处理THESC细胞，以触发炎症反应。炎症基因(如肿瘤坏死因子α)的表达将通过实时荧光聚合酶链式反应(Real-time PCR)进行检测。本研究的目的是确定匹罗卡品治疗是否能预防或减轻子宫内膜间质细胞对巨噬细胞条件培养液的炎症反应。利兹·霍夫曼在2010年夏天得到了ARRA 4000美元的资金支持，实验室获得了1000美元。2010年夏天，DWU的一名本科生与艾斯特博士共事。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Endometriosis is an inflammatory disease, and cells of endometriosis explants exhibit invasive properties. Both the inflammatory response and the invasive response can be mimicked by treatments of endometrial stromal cells in culture. This project will examine the anti-inflammatory effects of parasympathetic nervous system mediators on endometrial stromal cells in culture. The laboratory has identified a signature of inflammatory genes expressed in THESC cells in response to treatment with macrophage conditioned medium. THESC cells will be treated with macrophage conditioned medium to trigger the inflammatory response in the absence and presence of the muscarinic receptor agonist, pilocarpine. The expression of inflammatory genes (such as tumor necrosis factor alpha) will be measured by real-time PCR. The goal of the study is to determine whether pilocarpine treatment can prevent or reduce the inflammatory response to endometrial stromal cells to macrophage conditioned medium. Liz Hoffman was supported by $4000 in ARRA funds during the summer of 2010 and the lab received $1000. An undergraduate fellow from DWU worked with Dr. Eyster during the summer of 2010.