CEST EFFECT FROM PHOSPHO-CREATINE (PCR) AND ADENOSINE-TRI-PHOSPHATE (ATP)

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 磷酸肌酸(PCr)和三磷酸腺苷(ATP)是生物系统中的主要能源/蓄水池。不同器官中的三磷酸腺苷和聚合酶链式反应的浓度不同。脑、心肌和肌肉中的ATP浓度分别为~3~4 mM、~6 mM和~10 mM。而PCR5-6 mm，心脏10 mm，肌肉33.5 mm，是能量储备的主要来源。据报道，这些富含能量的化合物在各种疾病中的变化，包括肿瘤、心肌梗死和肌肉疾病。~(31)P-MRS已被广泛用于检测这些代谢物的信号。先前已报道过利用PI和PCR之间的MT效应进行聚合酶链式反应的磷成像。在这里，我们首次报道了通过利用模体的不稳定胺质子(-NH2)和静止的小腿肌肉的PCRMRI CEST图谱，在体外对模体进行化学交换饱和转移(CEST)的研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phospho-creatine (PCr) and Adenosine tri-phosphate (ATP) are the major energy sources/reservoirs in the biological system. The concentration of ATP and PCr is varied in the different organs. The ATP concentration in brain, heart and muscle is ~3-4mM, ~6mM and ~10mM, respectively. On the other hand PCr which is a major source of energy reservoir present around 5-6mM in brain, 10mM in heart and around 33.5mM in muscles. Alternations of these energy-rich compounds have been reported in various disease conditions including tumors, heart infarction and muscular disorders. 31P MRS has been widely used to detect the signal from these metabolites. Phosphorous imaging of PCr has been previously reported utilizing MT effects between Pi and PCr. Here, for the first time we report the chemical exchange saturation transfer (CEST) effect from PCr and ATP in-vitro on phantoms by exploiting their labile amine protons (-NH2) as well as a PCr MRI CEST map from resting calf muscle.