OVARIAN CANCER PLATELETS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Sood实验室的最新发现表明，血小板可能在卵巢癌的生长和转移中发挥重要作用。我们假设，血小板超微结构的变化反映了促进卵巢癌患者疾病进展的血小板功能的变化。因此，为了更好地了解卵巢癌中可能指示血小板病理生物学的超微结构变化，我们建议使用电子冷冻显微镜在超微结构水平上比较从健康献血者和新诊断的卵巢癌患者的外周血中分离出的血小板。现在临床上使用血小板电子显微镜来帮助确定患者是否存在与出血风险相关的固有血小板异常。电子冷冻显微镜的固有优势使其更适合于目前的研究，因为冷冻-EM将提供更好的样品保存。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent findings from The Sood laboratory indicate that platelets may play an important role in ovarian cancer growth and metastasis. We hypothesize that platelet ultra-structural changes reflect alterations in platelet function that facilitate disease progression in ovarian cancer patients. Thus, in order to gain better understanding of ultra-structural changes that may denote platelet pathobiology in ovarian cancer, we propose comparing platelets isolated from the peripheral blood of healthy donors to those from newly diagnosed ovarian cancer patients at the ultrastructural level using electron cryomicroscopy. Platelet electron microscopy is now used clinically to help determine if patients have an intrinsic platelet abnormality related to bleeding risk. The inherent advantages of electron cryomicroscopy make it preferable for the current investigation because cryo-EM would afford improved sample preservation.