ANNOTATION OF PROTEIN FUNCTION BY LIGAND DESCRIPTORS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 生物学中的一个关键问题是为已知的蛋白质分配功能。 序列，有时甚至是已知的结构，但未知的活动。 例子包括努力去孤儿受体，确定目标， 基于细胞的（功能）筛选和结构基因组学。多的努力 一直专注于生物信息学工具，但 化学信息在很大程度上被忽视了。许多蛋白质识别 有机分子（配体）的特征集，无论是在其 正常功能或作为中断该功能的试剂。我们 建议使用蛋白质的配体来“识别”蛋白质。基于 这种指纹与其他蛋白质的指纹相似， 可以创建基于配体化学的连锁图谱，并推断 蛋白质功能

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A key problem in biology is assigning function to proteins of known sequence, sometimes even known structures, but unknown activity. Examples include efforts to de-orphan receptors, determine targets in cell-based (functional) screens, and structural genomics. Much effort has been focused on bioinformatics tools, but a possible role for chemical information has largely been ignored. Many proteins recognize characteristic sets of organic molecules (ligands), either in their normal function or as reagents that interrupt this function. We propose to "fingerprint" proteins using their ligands. Based on the similarity of this fingerprint to those of other proteins, it should be possible to create ligand chemistry-based linkage maps and to infer protein function.