CRYSTALLOGRAPHIC STUDIES OF RECEPTORS OF NEUROLOGICAL AND IMMUNOLOGICAL RELEVANC

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我的实验室对与人类健康和疾病相关的细胞表面受体/配体相互作用的结构方面感兴趣。通过结构分析，我们希望回答有关跨膜信号传导的基本机制问题，并产生可用作药物设计模板的结构数据。我们从事许多糖蛋白受体的表达和结晶研究，包括单独的和与其配体的复合物。我们表达可溶性受体结构域以及全长整合膜蛋白。我们研究的受体系统主要在免疫和神经系统中，特别强调理解分子识别中的交叉反应性。我们的大多数项目都严重依赖于在同步加速器设施中收集X射线数据的能力，因为我们可以生长大型和异质蛋白质复合物的晶体通常较小且质量适中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. My laboratory is interested in structural aspects of cell-surface receptor/ligand interactions with relevance to human health and disease. Through structural analysis we hope to answer basic mechanistic questions about transmembrane signaling, as well as produce structural data that can be used as a template for drug design. We are engaged in expression and crystallization studies of numerous glycoprotein receptors both alone and in complex with their ligands. We are expressing both soluble receptor domains as well as full-length integral membrane proteins. The receptor systems we study are primarily in the immune and nervous systems, with a special emphasis on understanding cross-reactivity in molecular recognition. Most of our projects we are critically dependent on the ability to collect x-ray data at a synchrotron facility due to the generally small and moderate quality of the crystals which we can grow of large and hetero-geneous protein complexes.