NATIVE ESI FOR DETERMINING STOICHIOMETRY OF MULTISUBUNIT COMPLEXES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 光合作用是一个重要的生物过程，它产生人类所需的所有食物和大部分能量。人们已经做了大量的工作来了解光收集的结构基础和能量转移过程的效率。几种细菌捕光（LH）复合物，如LH 1、LH 2和Fenna-Matthews-Olson蛋白（FMO），已经作为结构、光谱和理论研究的模型系统。最近发现的嗜热细菌--嗜酸绿杆菌（CordatusChloracidobacterium（C.）嗜热菌（thermophilum），属于酸细菌门，并且是迄今为止在该门中发现的唯一的绿色光合生物。令人惊讶的是，好氧C.嗜热菌非常类似于严格厌氧的绿色硫细菌（GSB）。然而，使C.嗜热菌细胞在需氧条件下进行光养的能力尚不清楚。绿色硫细菌（绿藻目）的FMO蛋白已被广泛研究，使用广泛的光谱和理论方法。通过几种方法研究新的FMO蛋白，包括在Bruker 12特斯拉FTICR质谱仪中的天然电喷雾。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Photosynthesis is a central biological process that produces all the food and much of the energy used by human beings. An enormous amount of work has been done to understand the structural basis of light harvesting and the efficiency of the energy-transfer process. Several bacterial light-harvesting (LH) complexes, such as LH1, LH2 and Fenna-Matthews-Olson protein (FMO) have served as model systems for structural, spectroscopic and theoretical studies.. The recently discovered, thermophilic bacterium, Candidatus Chloracidobacterium (C.) thermophilum, belongs to the phylum Acidobacteria, and is the only chlorophototroph found in the phylum so far. Surprisingly, the photosystem of the aerobic C. thermophilum closely resembles that of the green sulfur bacteria (GSB), which are strict anaerobes. However, The mechanism that enables the C. thermophilum cells to carry out phototrophy under aerobic conditions is not yet known. FMO proteins of green sulfur bacteria (Chlorobiales) have been extensively studied using a wide range of spectroscopic and theoretical approaches. The new FMO protein is investigated by several approaches including native electrospray in a Bruker 12 tesla FTICR mass spectrometer.