DYNAMICS OF VIROLOGICAL SYNAPSES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们希望使用荧光漂白后恢复(FRAP)来测量当接触到gp120蛋白或金纳米颗粒标记的受体抗体等配体时，荧光标记的受体CD4和CCR5在细胞膜上的侧向扩散速率。我们已经瞬时地将表达这些蛋白质的细胞导入细胞，并在对它们进行成像之前不久将它们暴露在配体中。我们希望测量这些受体在与这些配体结合时扩散动力学的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We wish to use fluorescent recovery after photobleaching (FRAP) to measure lateral diffusion rates of fluorescently labeled receptors CD4 and CCR5 on cell membranes when exposed to ligands such as gp120 protein or gold nanoparticle-tagged antibodies to the receptors. We have transiently transfected cells expressing these proteins and we would expose them to the ligands shortly before imaging them. We wish to measure the change in diffusion dynamics of these receptors when bound to these ligands.