TRP CHANNEL MODULATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个项目的目的是对调节痛觉重要通道活动的蛋白质进行生化表征。研究的对象将包括通道胞质结构域、小分子调节器、多肽调节器以及相关的膜或细胞质蛋白。其目的是纯化蛋白质，确定分子相互作用部位，并进行结构研究。加州大学旧金山分校的质谱学设备将帮助我们确定纯化产品的准确质量和含量，以及确认纯化产品的身份。在该设施的帮助下，我们还可以在未来解释化学交联研究的结果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The purpose of this project is to perform biochemical characterization of proteins that modulate the activity of channels important for pain sensation. The targets of study will include channel cytoplasmic domains, small molecule modulators, peptide modulators, and associated membrane or cytoplasmic proteins. The objective is to purify proteins, determine molecular interaction sites, and perform structural studies.The UCSF mass spectrometry facility will aid us in determining the accurate mass and of purified products, as well as confirming the identity of purified products. The aid of the facility will also allow us to interpret results from chemical crosslinking studies in the future.