UNDERSTANDING THE CHROMOSOMAL SEGROSOME ORGANIZATION IN MYCOBACTERIUM TUBERCULOS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们建议研究组织的segrosome，细胞器的染色体分离在人类病原体结核分枝杆菌。两种蛋白质（帕拉和ParB）和一组原始近端DNA序列（parS）形成了分离体机制。帕拉是驱动这一过程的马达蛋白。ParB与parS DNA序列形成核蛋白复合物，并粘附在parS近端DNA支架上以形成超结构。我们的目的是阐明M.结核从SSRL获得的X射线溶液散射数据（SAXS）将与额外的实验数据和同源建模方法相结合，以建立ParB-parS复合物和核蛋白丝的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We propose to study the organization of segrosome, the cellular apparatus for chromosome segregation in human pathogen Mycobacterium tuberculosis. Two proteins (ParA and ParB) and a set of original-proximal DNA sequences (parS) form the segrosome machinery. ParA is a motor protein that drives this process. ParB forms a nucleo-protein complex with the parS DNA sequence and polymerize on the parS-proximal DNA scaffold to form a superstructure. Our aim is to elucidate the three-dimensional organization of this nucleoprotein complex for the segrosome of M. tuberculosis. X-ray solution scattering data (SAXS) obtained from SSRL will be combined with additional experimental data and homology modeling approach to build a model of the ParB-parS complex and the nucleoprotein filament.